Detailed information of TA system TA116029

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 6178226..6178794
• Replicon: chromosome
• Accession: NZ_CP034436
• Organism: Pseudomonas aeruginosa strain B17932

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A0D0R3P1
• Locus tag: EJP73_RS29390
• Protein ID: WP_011222050.1
• Coordinates: 6178226..6178519 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A6M3UV67
• Locus tag: EJP73_RS29395
• Protein ID: WP_011222051.1
• Coordinates: 6178507..6178794 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJP73_RS29355	6173474..6174373	+	900	WP_032494864.1	class A extended-spectrum beta-lactamase VEB-9	-
EJP73_RS29360	6175107..6176297	-	1191	WP_033991588.1	tetracycline efflux MFS transporter Tet(A)	-
EJP73_RS29365	6176403..6177053	+	651	WP_000164043.1	tetracycline resistance transcriptional repressor TetR(A)	-
EJP73_RS29370	6177085..6177327	-	243	WP_000844627.1	transposase	-
EJP73_RS29380	6177756..6177941	+	186	Protein_5643	multidrug transporter	-
EJP73_RS32230	6177977..6178288	+	312	WP_052150402.1	hypothetical protein	-
EJP73_RS29390	6178226..6178519	-	294	WP_011222050.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EJP73_RS29395	6178507..6178794	-	288	WP_011222051.1	CopG family ribbon-helix-helix protein	Antitoxin
EJP73_RS29400	6178967..6179173	-	207	WP_033991586.1	hypothetical protein	-
EJP73_RS29405	6179408..6181087	+	1680	WP_000179844.1	DDE-type integrase/transposase/recombinase	-
EJP73_RS29410	6181090..6182082	+	993	WP_001381192.1	TniB family NTP-binding protein	-
EJP73_RS29415	6182051..6182551	-	501	WP_000376623.1	GNAT family N-acetyltransferase	-
EJP73_RS29425	6182679..6183518	-	840	WP_000259031.1	sulfonamide-resistant dihydropteroate synthase Sul1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	sul1 / blaVEB-1 / tet(A) / ant(3'')-Ia / blaOXA-10	-	6159119..6229514	70395
	-	flank	IS/Tn	sul1 / ant(3'')-Ia / blaOXA-10	-	6182679..6187013	4334

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-94)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-45)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10890.71 Da
• Isoelectric Point: 10.8518

>T116029 WP_011222050.1 NZ_CP034436:c6178519-6178226 [Pseudomonas aeruginosa]
MPQVILSPAALRDLKRLREFLHSKSPVVAQRAAQTIRSALAQLGQQPAMGRPIEGLPEVFREWVIPFGDSGYLARYRLEG
ETVVILALRHQREAGFI

Nucleotide

• Length: 294 bp

>T116029 NZ_CP034436:c6178519-6178226 [Pseudomonas aeruginosa]
ATGCCACAAGTGATCCTCTCTCCGGCTGCTCTGCGTGACCTTAAACGGCTGCGCGAGTTCTTGCACAGTAAGAGCCCAGT
TGTTGCACAGCGAGCAGCCCAAACCATCCGTTCGGCGCTGGCCCAGCTCGGGCAACAACCCGCGATGGGGCGTCCTATTG
AAGGGCTCCCGGAGGTGTTCCGCGAGTGGGTCATCCCCTTTGGGGACAGTGGTTATCTGGCGCGTTACCGCCTAGAGGGC
GAAACCGTAGTGATCTTGGCTCTGCGTCACCAGCGCGAAGCCGGGTTTATTTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10922.19 Da
• Isoelectric Point: 5.1897

>AT116029 WP_011222051.1 NZ_CP034436:c6178794-6178507 [Pseudomonas aeruginosa]
MSAATKPMSIKLDSDTQSRIKQLAAARDRSAHWMMREAITQYVEREERKEAFRRDTLDAWQEFQETGLHVSLEETDQWLA
GWGTEIEGGAPACHK

Nucleotide

• Length: 288 bp

>AT116029 NZ_CP034436:c6178794-6178507 [Pseudomonas aeruginosa]
ATGTCCGCAGCTACCAAACCCATGTCTATCAAACTTGATAGCGATACCCAAAGCCGGATCAAGCAGCTAGCTGCTGCCCG
CGACCGTTCAGCGCACTGGATGATGCGCGAAGCTATCACCCAGTATGTTGAGCGCGAAGAGCGCAAGGAGGCGTTCCGCC
GCGATACCCTCGATGCTTGGCAGGAGTTTCAGGAGACTGGTTTGCATGTCTCTCTTGAGGAGACCGATCAGTGGTTGGCT
GGTTGGGGCACCGAGATTGAAGGTGGTGCACCTGCATGCCACAAGTGA

Structures

Toxin

• AlphaFold DB: A0A0D0R3P1

Antitoxin

• AlphaFold DB: A0A6M3UV67