Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 1310800..1311408 Replicon chromosome
Accession NZ_CP034436
Organism Pseudomonas aeruginosa strain B17932

Toxin (Protein)


Gene name PfiT Uniprot ID A0A444LUU5
Locus tag EJP73_RS06075 Protein ID WP_019486378.1
Coordinates 1311061..1311408 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag EJP73_RS06070 Protein ID WP_003114155.1
Coordinates 1310800..1311051 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJP73_RS06050 1306447..1306803 + 357 WP_003114150.1 DUF2523 domain-containing protein -
EJP73_RS06055 1306807..1308081 + 1275 WP_033990855.1 zonular occludens toxin family protein -
EJP73_RS06060 1308311..1309603 + 1293 WP_033990854.1 hypothetical protein -
EJP73_RS06065 1309603..1310586 + 984 WP_033990852.1 tyrosine-type recombinase/integrase -
EJP73_RS06070 1310800..1311051 + 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EJP73_RS06075 1311061..1311408 + 348 WP_019486378.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EJP73_RS06085 1311735..1312637 - 903 WP_003085447.1 (R)-3-hydroxydecanoyl-ACP:CoA transacylase -
EJP73_RS06090 1313089..1313826 - 738 WP_003085453.1 murein L,D-transpeptidase catalytic domain family protein -
EJP73_RS06095 1313906..1314949 - 1044 WP_003134392.1 L,D-transpeptidase -
EJP73_RS06100 1315085..1315777 - 693 WP_003085458.1 16S rRNA pseudouridine(516) synthase -
EJP73_RS06105 1315777..1316049 - 273 WP_074241374.1 cysteine-rich CWC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1296581..1311408 14827


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12956.72 Da        Isoelectric Point: 4.4212

>T116024 WP_019486378.1 NZ_CP034436:1311061-1311408 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEAAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T116024 NZ_CP034436:1311061-1311408 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGCGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT116024 WP_003114155.1 NZ_CP034436:1310800-1311051 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT116024 NZ_CP034436:1310800-1311051 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A444LUU5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References