Detailed information of TA system TA116023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 911338..911924
• Replicon: chromosome
• Accession: NZ_CP034436
• Organism: Pseudomonas aeruginosa strain B17932

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: EJP73_RS04180
• Protein ID: WP_003120987.1
• Coordinates: 911338..911637 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: EJP73_RS04185
• Protein ID: WP_003448662.1
• Coordinates: 911634..911924 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJP73_RS04160	906451..906984	-	534	WP_004352829.1	type IV pilus biogenesis protein PilP	-
EJP73_RS04165	906974..908299	-	1326	WP_022579625.1	type 4b pilus protein PilO2	-
EJP73_RS04170	908303..910012	-	1710	WP_023093673.1	PilN family type IVB pilus formation outer membrane protein	-
EJP73_RS04175	910012..911136	-	1125	WP_023093672.1	TcpQ domain-containing protein	-
EJP73_RS04180	911338..911637	+	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EJP73_RS04185	911634..911924	+	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
EJP73_RS04190	912007..912342	-	336	WP_022579623.1	hypothetical protein	-
EJP73_RS04195	912481..914457	-	1977	WP_022579622.1	DEAD/DEAH box helicase	-
EJP73_RS04200	914454..916343	-	1890	WP_033989533.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	911338..939538	28200

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T116023 WP_003120987.1 NZ_CP034436:911338-911637 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T116023 NZ_CP034436:911338-911637 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT116023 WP_003448662.1 NZ_CP034436:911634-911924 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT116023 NZ_CP034436:911634-911924 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
CTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCTCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCGACATTGAAAACCATGCTGGCA
GTAATGCGAGCTTTGGGCGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73