116014

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 4705944..4706552 Replicon chromosome
Accession NZ_CP034429
Organism Pseudomonas aeruginosa strain GIMC5015:PAKB6

Toxin (Protein)


Gene name PfiT Uniprot ID Q9I5J9
Locus tag PAKB6_RS22335 Protein ID WP_003114156.1
Coordinates 4705944..4706291 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag PAKB6_RS22340 Protein ID WP_003114155.1
Coordinates 4706301..4706552 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PAKB6_RS22305 4701303..4701575 + 273 WP_010895521.1 cysteine-rich CWC family protein -
PAKB6_RS22310 4701575..4702267 + 693 WP_003114159.1 16S rRNA pseudouridine(516) synthase -
PAKB6_RS22315 4702403..4703446 + 1044 WP_003098363.1 L,D-transpeptidase -
PAKB6_RS22320 4703526..4704263 + 738 WP_003114158.1 murein L,D-transpeptidase catalytic domain family protein -
PAKB6_RS22325 4704715..4705617 + 903 WP_003114157.1 (R)-3-hydroxydecanoyl-ACP:CoA transacylase -
PAKB6_RS22335 4705944..4706291 - 348 WP_003114156.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PAKB6_RS22340 4706301..4706552 - 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
PAKB6_RS22345 4706766..4707749 - 984 WP_003114154.1 tyrosine-type recombinase/integrase -
PAKB6_RS22350 4707749..4709041 - 1293 WP_003115206.1 hypothetical protein -
PAKB6_RS22355 4709271..4710545 - 1275 WP_010895520.1 zonular occludens toxin family protein -
PAKB6_RS22360 4710549..4710905 - 357 WP_003114150.1 DUF2523 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage catB7 - 4705944..4728065 22121


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12984.78 Da        Isoelectric Point: 4.4212

>T116014 WP_003114156.1 NZ_CP034429:c4706291-4705944 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T116014 NZ_CP034429:c4706291-4705944 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT116014 WP_003114155.1 NZ_CP034429:c4706552-4706301 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT116014 NZ_CP034429:c4706552-4706301 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9I5J9

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References