Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 81866..82509 Replicon plasmid pKPC-CR-hvKP-C789
Accession NZ_CP034417
Organism Klebsiella pneumoniae strain C789

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag EJJ25_RS28280 Protein ID WP_001044770.1
Coordinates 81866..82282 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag EJJ25_RS28285 Protein ID WP_001261282.1
Coordinates 82279..82509 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJJ25_RS28260 77969..78241 - 273 Protein_102 transposase -
EJJ25_RS28265 78270..78967 - 698 Protein_103 IS1 family transposase -
EJJ25_RS28270 79223..80245 - 1023 WP_000361404.1 helicase UvrD -
EJJ25_RS28275 80230..81792 - 1563 WP_004206609.1 AAA family ATPase -
EJJ25_RS28280 81866..82282 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
EJJ25_RS28285 82279..82509 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
EJJ25_RS29970 82466..82927 + 462 WP_014343465.1 hypothetical protein -
EJJ25_RS28290 83088..84032 + 945 WP_011977810.1 hypothetical protein -
EJJ25_RS28295 84069..84461 + 393 WP_011977811.1 hypothetical protein -
EJJ25_RS28300 84519..85040 + 522 WP_013214008.1 hypothetical protein -
EJJ25_RS28305 85086..85289 + 204 WP_011977813.1 hypothetical protein -
EJJ25_RS28310 85319..86323 + 1005 WP_011977814.1 hypothetical protein -
EJJ25_RS28315 86507..87286 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2 - 1..128299 128299


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T116000 WP_001044770.1 NZ_CP034417:c82282-81866 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T116000 NZ_CP034417:c82282-81866 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT116000 WP_001261282.1 NZ_CP034417:c82509-82279 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT116000 NZ_CP034417:c82509-82279 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References