Detailed information of TA system TA115995

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 142699..143369
• Replicon: plasmid pVir-CR-hvKP-C789
• Accession: NZ_CP034416
• Organism: Klebsiella pneumoniae strain C789

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: EJJ25_RS27360
• Protein ID: WP_004213072.1
• Coordinates: 142699..143142 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: EJJ25_RS27365
• Protein ID: WP_004213073.1
• Coordinates: 143139..143369 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJJ25_RS27325	138110..138385	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
EJJ25_RS27330	138448..138939	+	492	WP_011251327.1	DM13 domain-containing protein	-
EJJ25_RS27335	138988..139908	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
EJJ25_RS27340	139999..140405	+	407	Protein_148	GAF domain-containing protein	-
EJJ25_RS30285	140920..141555	-	636	Protein_149	response regulator	-
EJJ25_RS27350	141972..142265	+	294	Protein_150	transposase	-
EJJ25_RS27355	142269..142685	+	417	Protein_151	restriction endonuclease	-
EJJ25_RS27360	142699..143142	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
EJJ25_RS27365	143139..143369	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EJJ25_RS27370	143977..145110	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
EJJ25_RS27375	145126..145419	+	294	WP_004213076.1	hypothetical protein	-
EJJ25_RS27380	145409..145615	-	207	WP_004213077.1	hypothetical protein	-
EJJ25_RS27385	145967..146257	+	291	WP_004213078.1	hypothetical protein	-
EJJ25_RS27390	146247..147146	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..215950	215950

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T115995 WP_004213072.1 NZ_CP034416:c143142-142699 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T115995 NZ_CP034416:c143142-142699 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT115995 WP_004213073.1 NZ_CP034416:c143369-143139 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT115995 NZ_CP034416:c143369-143139 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620