Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4815846..4816465 Replicon chromosome
Accession NZ_CP034408
Organism Klebsiella pneumoniae strain NH34

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag EJC76_RS26300 Protein ID WP_002892050.1
Coordinates 4815846..4816064 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag EJC76_RS26305 Protein ID WP_002892066.1
Coordinates 4816091..4816465 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJC76_RS26270 4811891..4812154 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
EJC76_RS26275 4812154..4812294 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
EJC76_RS26280 4812291..4812989 - 699 WP_004178762.1 GNAT family N-acetyltransferase -
EJC76_RS26285 4813090..4814541 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
EJC76_RS26290 4814516..4814986 - 471 WP_002892026.1 YlaC family protein -
EJC76_RS30175 4815007..4815147 + 141 WP_004147370.1 hypothetical protein -
EJC76_RS26295 4815119..4815687 - 569 Protein_4573 maltose O-acetyltransferase -
EJC76_RS26300 4815846..4816064 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
EJC76_RS26305 4816091..4816465 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
EJC76_RS26310 4816952..4820098 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
EJC76_RS26315 4820121..4821314 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T115962 WP_002892050.1 NZ_CP034408:c4816064-4815846 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T115962 NZ_CP034408:c4816064-4815846 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT115962 WP_002892066.1 NZ_CP034408:c4816465-4816091 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT115962 NZ_CP034408:c4816465-4816091 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References