Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/ElaA-DUF1778
Location 276937..277688 Replicon plasmid pNH34.1
Accession NZ_CP034406
Organism Klebsiella pneumoniae strain NH34

Toxin (Protein)


Gene name ataT Uniprot ID H6U1U8
Locus tag EJC76_RS01635 Protein ID WP_014386536.1
Coordinates 277206..277688 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A071LPN3
Locus tag EJC76_RS01630 Protein ID WP_004902250.1
Coordinates 276937..277215 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJC76_RS01595 272580..273005 - 426 WP_000522996.1 organomercurial transporter MerC -
EJC76_RS01600 273033..273308 - 276 WP_000732275.1 mercury resistance system periplasmic binding protein MerP -
EJC76_RS01605 273324..273689 - 366 WP_004200999.1 mercuric ion transporter MerT -
EJC76_RS01610 273761..274216 + 456 WP_001166628.1 Hg(II)-responsive transcriptional regulator -
EJC76_RS01615 274869..275327 + 459 WP_014386535.1 hypothetical protein -
EJC76_RS01620 276157..276498 + 342 WP_004902257.1 hypothetical protein -
EJC76_RS01625 276606..276818 + 213 WP_004902255.1 hypothetical protein -
EJC76_RS01630 276937..277215 + 279 WP_004902250.1 DUF1778 domain-containing protein Antitoxin
EJC76_RS01635 277206..277688 + 483 WP_014386536.1 GNAT family N-acetyltransferase Toxin
EJC76_RS01650 278723..279262 + 540 WP_004902239.1 hypothetical protein -
EJC76_RS01655 279367..279759 + 393 WP_032442757.1 hypothetical protein -
EJC76_RS01660 279860..280615 + 756 WP_004902235.1 DUF2971 domain-containing protein -
EJC76_RS01665 280642..281289 + 648 WP_014386537.1 EcsC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaOXA-1 / aac(6')-Ib-cr / qnrB1 / dfrA14 / tet(D) / blaTEM-1A / aac(6')-Ib / blaCTX-M-15 / dfrA12 / aadA2 / qacE / sul1 / armA / msr(E) / mph(E) / aph(3')-VI / blaNDM-1 - 1..300327 300327


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(42-144)

Antitoxin

Vibrio phage ICP1, Orf50

(10-86)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17662.45 Da        Isoelectric Point: 8.9130

>T115946 WP_014386536.1 NZ_CP034406:277206-277688 [Klebsiella pneumoniae]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Download         Length: 483 bp

>T115946 NZ_CP034406:277206-277688 [Klebsiella pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10704.23 Da        Isoelectric Point: 4.5271

>AT115946 WP_004902250.1 NZ_CP034406:276937-277215 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Download         Length: 279 bp

>AT115946 NZ_CP034406:276937-277215 [Klebsiella pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MBI1

Antitoxin

Source ID Structure
AlphaFold DB A0A071LPN3

References