115945

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 198981..199503 Replicon plasmid pNH34.1
Accession NZ_CP034406
Organism Klebsiella pneumoniae strain NH34

Toxin (Protein)


Gene name stbE Uniprot ID A0A0C7KG00
Locus tag EJC76_RS01150 Protein ID WP_038991638.1
Coordinates 198981..199265 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4R0U8
Locus tag EJC76_RS01155 Protein ID WP_004181777.1
Coordinates 199255..199503 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJC76_RS01120 194181..195276 + 1096 Protein_204 conjugal transfer protein TraG N-terminal domain-containing protein -
EJC76_RS01125 195375..196562 - 1188 WP_043907031.1 transposase -
EJC76_RS01130 196598..197026 + 429 WP_077256229.1 IS200/IS605 family transposase -
EJC76_RS01135 197105..197329 + 225 Protein_207 transposase -
EJC76_RS01140 197601..198827 - 1227 WP_080030933.1 transposase -
EJC76_RS01145 198863..198964 + 102 Protein_209 IS200/IS605 family transposase -
EJC76_RS01150 198981..199265 - 285 WP_038991638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EJC76_RS01155 199255..199503 - 249 WP_004181777.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EJC76_RS01160 199794..201593 + 1800 WP_043907032.1 ATP-dependent helicase -
EJC76_RS01165 201927..202913 + 987 WP_040120328.1 hypothetical protein -
EJC76_RS01170 203058..203411 + 354 WP_004181774.1 hypothetical protein -
EJC76_RS01175 203473..204294 + 822 WP_004181772.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaOXA-1 / aac(6')-Ib-cr / qnrB1 / dfrA14 / tet(D) / blaTEM-1A / aac(6')-Ib / blaCTX-M-15 / dfrA12 / aadA2 / qacE / sul1 / armA / msr(E) / mph(E) / aph(3')-VI / blaNDM-1 - 1..300327 300327


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10993.73 Da        Isoelectric Point: 10.6516

>T115945 WP_038991638.1 NZ_CP034406:c199265-198981 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYHLTKHRN

Download         Length: 285 bp

>T115945 NZ_CP034406:c199265-198981 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACCATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8925.17 Da        Isoelectric Point: 3.8209

>AT115945 WP_004181777.1 NZ_CP034406:c199503-199255 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT115945 NZ_CP034406:c199503-199255 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGTTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C7KG00

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R0U8

References