115944

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 56138..57048 Replicon plasmid pNH34.1
Accession NZ_CP034406
Organism Klebsiella pneumoniae strain NH34

Toxin (Protein)


Gene name - Uniprot ID A0A5Q9LR54
Locus tag EJC76_RS00305 Protein ID WP_004181896.1
Coordinates 56138..56608 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag EJC76_RS00310 Protein ID WP_004181895.1
Coordinates 56605..57048 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJC76_RS00275 51634..52011 + 378 WP_004181901.1 hypothetical protein -
EJC76_RS30300 52209..53183 - 975 WP_043907013.1 hypothetical protein -
EJC76_RS00290 53786..53944 - 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
EJC76_RS00295 54016..55197 + 1182 WP_004883380.1 IS481 family transposase -
EJC76_RS00300 55768..56028 + 261 WP_004026352.1 hypothetical protein -
EJC76_RS00305 56138..56608 - 471 WP_004181896.1 RES family NAD+ phosphorylase Toxin
EJC76_RS00310 56605..57048 - 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
EJC76_RS00315 57149..58420 - 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
EJC76_RS00320 58420..58842 - 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
EJC76_RS00325 60027..61058 - 1032 WP_170856552.1 CRISPR-associated protein Csf2 -
EJC76_RS00330 61063..61770 - 708 WP_062826779.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaOXA-1 / aac(6')-Ib-cr / qnrB1 / dfrA14 / tet(D) / blaTEM-1A / aac(6')-Ib / blaCTX-M-15 / dfrA12 / aadA2 / qacE / sul1 / armA / msr(E) / mph(E) / aph(3')-VI / blaNDM-1 - 1..300327 300327
- flank IS/Tn - - 54016..55197 1181


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17549.81 Da        Isoelectric Point: 4.6155

>T115944 WP_004181896.1 NZ_CP034406:c56608-56138 [Klebsiella pneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T115944 NZ_CP034406:c56608-56138 [Klebsiella pneumoniae]
GTGATCTTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT115944 WP_004181895.1 NZ_CP034406:c57048-56605 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT115944 NZ_CP034406:c57048-56605 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LR54

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References