Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 3915484..3916079 Replicon chromosome
Accession NZ_CP034404
Organism Escherichia coli strain CRE10

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag EJC75_RS20910 Protein ID WP_000239579.1
Coordinates 3915729..3916079 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag EJC75_RS20905 Protein ID WP_001223208.1
Coordinates 3915484..3915735 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJC75_RS20895 3911148..3914927 + 3780 WP_000060921.1 autotransporter assembly complex protein TamB -
EJC75_RS20900 3914930..3915271 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
EJC75_RS20905 3915484..3915735 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
EJC75_RS20910 3915729..3916079 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
EJC75_RS20915 3916159..3916689 - 531 WP_000055072.1 inorganic diphosphatase -
EJC75_RS20920 3916999..3917955 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
EJC75_RS20925 3918265..3919767 + 1503 WP_129484079.1 sugar ABC transporter ATP-binding protein -
EJC75_RS20930 3919781..3920803 + 1023 WP_024198507.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T115938 WP_000239579.1 NZ_CP034404:3915729-3916079 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T115938 NZ_CP034404:3915729-3916079 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT115938 WP_001223208.1 NZ_CP034404:3915484-3915735 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT115938 NZ_CP034404:3915484-3915735 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References