Detailed information of TA system TA115935

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3276559..3277393
• Replicon: chromosome
• Accession: NZ_CP034404
• Organism: Escherichia coli strain CRE10

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A4S3WCD6
• Locus tag: EJC75_RS17875
• Protein ID: WP_021566610.1
• Coordinates: 3277016..3277393 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B3Y195
• Locus tag: EJC75_RS17870
• Protein ID: WP_001285585.1
• Coordinates: 3276559..3276927 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJC75_RS26305	3273560..3273918	+	359	Protein_3065	hypothetical protein	-
EJC75_RS17840	3273997..3274230	+	234	WP_001119729.1	DUF905 family protein	-
EJC75_RS17850	3274330..3275148	+	819	WP_001234629.1	DUF945 domain-containing protein	-
EJC75_RS17855	3275230..3275709	+	480	WP_048265132.1	antirestriction protein	-
EJC75_RS17860	3275725..3276201	+	477	WP_001186726.1	RadC family protein	-
EJC75_RS17865	3276264..3276485	+	222	WP_000692323.1	DUF987 domain-containing protein	-
EJC75_RS17870	3276559..3276927	+	369	WP_001285585.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
EJC75_RS17875	3277016..3277393	+	378	WP_021566610.1	TA system toxin CbtA family protein	Toxin
EJC75_RS17880	3277390..3277878	+	489	WP_048237286.1	hypothetical protein	-
EJC75_RS17885	3277890..3278087	+	198	WP_000839269.1	DUF957 domain-containing protein	-
EJC75_RS17890	3278172..3279017	+	846	WP_021566612.1	DUF4942 domain-containing protein	-
EJC75_RS17900	3279809..3280993	+	1185	WP_001172876.1	sugar efflux transporter	-
EJC75_RS17905	3281104..3282027	+	924	WP_000535971.1	carboxylate/amino acid/amine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14058.02 Da
• Isoelectric Point: 7.8276

>T115935 WP_021566610.1 NZ_CP034404:3277016-3277393 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T115935 NZ_CP034404:3277016-3277393 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.48 Da
• Isoelectric Point: 6.3139

>AT115935 WP_001285585.1 NZ_CP034404:3276559-3276927 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT115935 NZ_CP034404:3276559-3276927 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A4S3WCD6

Antitoxin

• AlphaFold DB: A0A7U9LW60