115931

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1952580..1953205 Replicon chromosome
Accession NZ_CP034404
Organism Escherichia coli strain CRE10

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag EJC75_RS11380 Protein ID WP_000911329.1
Coordinates 1952580..1952978 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag EJC75_RS11385 Protein ID WP_000450524.1
Coordinates 1952978..1953205 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJC75_RS11360 1948458..1948658 + 201 WP_000383836.1 YpfN family protein -
EJC75_RS11365 1948768..1949466 - 699 WP_000679812.1 esterase -
EJC75_RS11370 1949540..1951555 - 2016 WP_000829332.1 tRNA cytosine(34) acetyltransferase TmcA -
EJC75_RS11375 1951570..1952433 - 864 WP_001267507.1 neutral zinc metallopeptidase -
EJC75_RS11380 1952580..1952978 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
EJC75_RS11385 1952978..1953205 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
EJC75_RS11390 1953361..1954074 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
EJC75_RS11395 1954287..1955321 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
EJC75_RS11400 1955338..1956216 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
EJC75_RS11405 1956362..1956934 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
EJC75_RS11410 1956934..1957404 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T115931 WP_000911329.1 NZ_CP034404:c1952978-1952580 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T115931 NZ_CP034404:c1952978-1952580 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAACATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT115931 WP_000450524.1 NZ_CP034404:c1953205-1952978 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT115931 NZ_CP034404:c1953205-1952978 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References