Detailed information of TA system TA115929

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1475902..1476740
• Replicon: chromosome
• Accession: NZ_CP034404
• Organism: Escherichia coli strain CRE10

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: E1IWA7
• Locus tag: EJC75_RS09165
• Protein ID: WP_000854751.1
• Coordinates: 1476363..1476740 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: EJC75_RS09160
• Protein ID: WP_129484035.1
• Coordinates: 1475902..1476273 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJC75_RS09125	1472738..1473193	+	456	WP_000581504.1	hypothetical protein	-
EJC75_RS09130	1473272..1473505	+	234	WP_001119729.1	DUF905 family protein	-
EJC75_RS09140	1473605..1474423	+	819	WP_001234630.1	DUF945 domain-containing protein	-
EJC75_RS09145	1474478..1474963	+	486	WP_000849582.1	antirestriction protein	-
EJC75_RS09150	1474979..1475455	+	477	WP_001186726.1	RadC family protein	-
EJC75_RS09155	1475518..1475739	+	222	WP_000692323.1	DUF987 domain-containing protein	-
EJC75_RS09160	1475902..1476273	+	372	WP_129484035.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EJC75_RS09165	1476363..1476740	+	378	WP_000854751.1	TA system toxin CbtA family protein	Toxin
EJC75_RS09170	1476737..1476940	+	204	WP_000761704.1	hypothetical protein	-
EJC75_RS09175	1476953..1477066	+	114	WP_001161660.1	DUF957 domain-containing protein	-
EJC75_RS09180	1477355..1477483	-	129	Protein_1407	transposase domain-containing protein	-
EJC75_RS09185	1477555..1477737	+	183	WP_001016348.1	hypothetical protein	-
EJC75_RS09190	1477838..1478167	-	330	WP_000450409.1	DUF496 family protein	-
EJC75_RS09195	1478339..1479397	-	1059	WP_001200910.1	FUSC family protein	-
EJC75_RS09200	1479595..1480068	-	474	WP_001105389.1	DNA gyrase inhibitor SbmC	-
EJC75_RS09205	1480187..1481353	-	1167	WP_001296209.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-118)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14050.04 Da
• Isoelectric Point: 8.2905

>T115929 WP_000854751.1 NZ_CP034404:1476363-1476740 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T115929 NZ_CP034404:1476363-1476740 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCCCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13604.53 Da
• Isoelectric Point: 6.6290

>AT115929 WP_129484035.1 NZ_CP034404:1475902-1476273 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTS
GELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 372 bp

>AT115929 NZ_CP034404:1475902-1476273 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCC
TGTTCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGC
GGTGAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAG
CGGTGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: E1IWA7