Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 56908..57509 Replicon plasmid pCRE10.1
Accession NZ_CP034401
Organism Escherichia coli strain CRE10

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag EJC75_RS00370 Protein ID WP_001216034.1
Coordinates 57129..57509 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag EJC75_RS00365 Protein ID WP_001190712.1
Coordinates 56908..57129 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJC75_RS00355 53889..55172 - 1284 WP_001617890.1 restriction endonuclease subunit S -
EJC75_RS00360 55169..56725 - 1557 WP_001553856.1 type I restriction-modification system subunit M -
EJC75_RS00365 56908..57129 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EJC75_RS00370 57129..57509 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
EJC75_RS00375 57514..57693 + 180 WP_001513661.1 hypothetical protein -
EJC75_RS00380 57721..58080 + 360 WP_001513660.1 hypothetical protein -
EJC75_RS00385 58004..58417 + 414 Protein_65 DDE-type integrase/transposase/recombinase -
EJC75_RS00395 59213..60190 + 978 WP_000361610.1 RepB family plasmid replication initiator protein -
EJC75_RS00400 60475..61215 - 741 WP_001066942.1 tyrosine-type recombinase/integrase -
EJC75_RS00405 61336..61518 - 183 WP_042065278.1 hypothetical protein -
EJC75_RS00415 61861..62168 - 308 Protein_69 type II toxin-antitoxin system ParD family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / catB3 / blaOXA-1 / aac(6')-Ib-cr / dfrA17 / aadA5 / qacE / mph(A) / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / aac(3)-IId - 1..134611 134611
- flank IS/Tn - - 58043..58417 374


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T115913 WP_001216034.1 NZ_CP034401:57129-57509 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T115913 NZ_CP034401:57129-57509 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTAGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT115913 WP_001190712.1 NZ_CP034401:56908-57129 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT115913 NZ_CP034401:56908-57129 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References