115911

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 37785..38310 Replicon plasmid pCRE10.1
Accession NZ_CP034401
Organism Escherichia coli strain CRE10

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag EJC75_RS00285 Protein ID WP_001159868.1
Coordinates 37785..38090 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A223LLB0
Locus tag EJC75_RS00290 Protein ID WP_023909027.1
Coordinates 38092..38310 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJC75_RS00270 33695..34861 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
EJC75_RS00275 35449..36204 - 756 WP_000852146.1 replication initiation protein RepE -
EJC75_RS00280 36978..37784 - 807 WP_000016982.1 site-specific integrase -
EJC75_RS00285 37785..38090 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
EJC75_RS00290 38092..38310 - 219 WP_023909027.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
EJC75_RS00295 38944..39141 + 198 WP_000215657.1 hypothetical protein -
EJC75_RS00300 39138..39422 - 285 WP_000642771.1 hypothetical protein -
EJC75_RS00305 39442..40575 - 1134 WP_000545983.1 DUF3800 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / catB3 / blaOXA-1 / aac(6')-Ib-cr / dfrA17 / aadA5 / qacE / mph(A) / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / aac(3)-IId - 1..134611 134611


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T115911 WP_001159868.1 NZ_CP034401:c38090-37785 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T115911 NZ_CP034401:c38090-37785 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.31 Da        Isoelectric Point: 4.7405

>AT115911 WP_023909027.1 NZ_CP034401:c38310-38092 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTIQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT115911 NZ_CP034401:c38310-38092 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATACAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A223LLB0

References