Detailed information of TA system TA115906

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 27151..27652
• Replicon: plasmid pCRE1.3
• Accession: NZ_CP034400
• Organism: Escherichia coli strain CRE1

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: H1ZXU8
• Locus tag: EJC48_RS25930
• Protein ID: WP_000702247.1
• Coordinates: 27151..27411 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: EJC48_RS25935
• Protein ID: WP_001132407.1
• Coordinates: 27401..27652 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJC48_RS25885	22748..22909	+	162	WP_000775236.1	type I toxin-antitoxin system Hok family toxin	-
EJC48_RS25890	23059..23655	+	597	WP_000125165.1	hypothetical protein	-
EJC48_RS26505	23673..23957	-	285	WP_001162371.1	hypothetical protein	-
EJC48_RS25900	24031..24696	-	666	WP_000532148.1	P-loop NTPase	-
EJC48_RS25910	25294..25623	+	330	WP_000855120.1	hypothetical protein	-
EJC48_RS25915	26205..26528	+	324	WP_000427403.1	hypothetical protein	-
EJC48_RS25920	26569..26778	+	210	WP_000866036.1	hypothetical protein	-
EJC48_RS25925	26875..27099	+	225	WP_000814381.1	hypothetical protein	-
EJC48_RS25930	27151..27411	+	261	WP_000702247.1	hypothetical protein	Toxin
EJC48_RS25935	27401..27652	+	252	WP_001132407.1	transcriptional regulator	Antitoxin
EJC48_RS25950	28202..28480	+	279	WP_000545938.1	hypothetical protein	-
EJC48_RS25955	28900..29166	+	267	WP_000681608.1	type II toxin-antitoxin system HicB family antitoxin	-
EJC48_RS25960	29273..29455	-	183	WP_001481829.1	hypothetical protein	-
EJC48_RS25965	30013..30195	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
EJC48_RS25970	30220..30657	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
EJC48_RS25975	30788..31114	-	327	WP_063078684.1	hypothetical protein	-
EJC48_RS25980	31134..31841	-	708	WP_021503228.1	hypothetical protein	-
EJC48_RS25985	31843..32595	-	753	WP_000797966.1	conjugal transfer protein TraL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..61553	61553

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9817.29 Da
• Isoelectric Point: 10.0665

>T115906 WP_000702247.1 NZ_CP034400:27151-27411 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRENDKEIVSMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T115906 NZ_CP034400:27151-27411 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT115906 WP_001132407.1 NZ_CP034400:27401-27652 [Escherichia coli]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT115906 NZ_CP034400:27401-27652 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A1P8DNC0