Detailed information of TA system TA115905

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 11366..11888
• Replicon: plasmid pCRE1.3
• Accession: NZ_CP034400
• Organism: Escherichia coli strain CRE1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YLU1
• Locus tag: EJC48_RS25790
• Protein ID: WP_000638823.1
• Coordinates: 11366..11647 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y7J1
• Locus tag: EJC48_RS25795
• Protein ID: WP_000121741.1
• Coordinates: 11637..11888 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJC48_RS25755	7515..7835	-	321	WP_000362080.1	VirB3 family type IV secretion system protein	-
EJC48_RS25760	7906..8094	-	189	WP_050567133.1	hypothetical protein	-
EJC48_RS25765	8196..8780	-	585	WP_129484133.1	lytic transglycosylase domain-containing protein	-
EJC48_RS25770	8801..9199	-	399	WP_001153665.1	hypothetical protein	-
EJC48_RS25775	9318..9755	-	438	WP_000539665.1	type IV pilus biogenesis protein PilM	-
EJC48_RS25780	9761..10996	-	1236	WP_015059538.1	TcpQ domain-containing protein	-
EJC48_RS25785	10999..11298	-	300	WP_000835764.1	IncN plasmid KikA protein	-
EJC48_RS25790	11366..11647	-	282	WP_000638823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EJC48_RS25795	11637..11888	-	252	WP_000121741.1	hypothetical protein	Antitoxin
EJC48_RS25800	11988..12623	-	636	WP_015059536.1	hypothetical protein	-
EJC48_RS25805	12696..12983	-	288	WP_001032611.1	EexN family lipoprotein	-
EJC48_RS25810	12996..13250	-	255	WP_001043555.1	EexN family lipoprotein	-
EJC48_RS25815	13252..13893	-	642	WP_001425343.1	conjugal transfer protein TrbJ	-
EJC48_RS25820	13899..14894	-	996	WP_001028543.1	type IV secretion system protein	-
EJC48_RS25825	14898..15155	-	258	WP_000739144.1	hypothetical protein	-
EJC48_RS25830	15152..15454	-	303	WP_001360345.1	hypothetical protein	-
EJC48_RS25835	15435..15692	-	258	WP_001542015.1	hypothetical protein	-
EJC48_RS25840	15725..16171	-	447	WP_001243165.1	hypothetical protein	-
EJC48_RS26350	16182..16352	-	171	WP_000550720.1	hypothetical protein	-
EJC48_RS25845	16356..16799	-	444	WP_000964330.1	NfeD family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..61553	61553

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10945.79 Da
• Isoelectric Point: 10.4997

>T115905 WP_000638823.1 NZ_CP034400:c11647-11366 [Escherichia coli]
MIYELAFDHRALKEWQKLGHTVREQFKKKLSERLINPRVPAAKLHGHTDRYKIKLRASGYRLVYQVIDEKVVLLVISVGK
REGSDVYHMADTR

Nucleotide

• Length: 282 bp

>T115905 NZ_CP034400:c11647-11366 [Escherichia coli]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCGTCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9223.38 Da
• Isoelectric Point: 3.9823

>AT115905 WP_000121741.1 NZ_CP034400:c11888-11637 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPELYAYYRELAEDAELNAIADERMKNPDIVRVNL
DDL

Nucleotide

• Length: 252 bp

>AT115905 NZ_CP034400:c11888-11637 [Escherichia coli]
ATGAGCTACCAAATTCTGACTACGACAGCAGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACTGTAGCCGAAGG
AGAAGGGGATGCCGTCGCAATCCTGAACCGAAATGAACCAGCGTTCTACTGTGTCCCACCAGAACTTTATGCCTACTACC
GGGAACTCGCTGAAGACGCTGAACTCAACGCAATTGCTGATGAAAGAATGAAAAATCCAGACATTGTAAGGGTTAACCTG
GATGATTTATGA

Structures

Toxin

• AlphaFold DB: U9YLU1

Antitoxin

• AlphaFold DB: U9Y7J1