Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 4613222..4613860 Replicon chromosome
Accession NZ_CP034399
Organism Escherichia coli strain CRE1

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag EJC48_RS24130 Protein ID WP_000813794.1
Coordinates 4613222..4613398 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag EJC48_RS24135 Protein ID WP_001270286.1
Coordinates 4613444..4613860 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJC48_RS24110 4608840..4610054 - 1215 WP_001307190.1 BenE family transporter YdcO -
EJC48_RS24115 4610107..4610644 + 538 Protein_4262 DNA-binding transcriptional regulator SutR -
EJC48_RS24120 4610717..4612678 + 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
EJC48_RS24125 4612770..4613000 - 231 WP_000494244.1 YncJ family protein -
EJC48_RS24130 4613222..4613398 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
EJC48_RS24135 4613444..4613860 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
EJC48_RS24140 4613939..4615345 + 1407 WP_000760589.1 PLP-dependent aminotransferase family protein -
EJC48_RS24145 4615590..4616735 + 1146 WP_000047455.1 ABC transporter substrate-binding protein -
EJC48_RS24150 4616753..4617766 + 1014 WP_000220416.1 ABC transporter ATP-binding protein -
EJC48_RS24155 4617767..4618708 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4607535..4608800 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T115904 WP_000813794.1 NZ_CP034399:4613222-4613398 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T115904 NZ_CP034399:4613222-4613398 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT115904 WP_001270286.1 NZ_CP034399:4613444-4613860 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT115904 NZ_CP034399:4613444-4613860 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References