Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3508413..3509250 Replicon chromosome
Accession NZ_CP034399
Organism Escherichia coli strain CRE1

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag EJC48_RS18780 Protein ID WP_000227784.1
Coordinates 3508413..3508955 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag EJC48_RS18785 Protein ID WP_001297137.1
Coordinates 3508939..3509250 (-) Length 104 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJC48_RS18760 3503952..3504863 - 912 WP_000705874.1 2-dehydropantoate 2-reductase -
EJC48_RS18765 3505031..3505522 + 492 WP_001138904.1 nucleotide binding protein YajQ -
EJC48_RS18770 3505650..3507014 - 1365 WP_001000978.1 MFS transporter -
EJC48_RS18775 3507422..3508357 + 936 WP_001297127.1 sel1 repeat family protein -
EJC48_RS18780 3508413..3508955 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
EJC48_RS18785 3508939..3509250 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
EJC48_RS18790 3509435..3510325 - 891 WP_000971336.1 heme o synthase -
EJC48_RS18795 3510337..3510666 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
EJC48_RS18800 3510666..3511280 - 615 WP_000179814.1 cytochrome o ubiquinol oxidase subunit III -
EJC48_RS18805 3511270..3513261 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
EJC48_RS18810 3513283..3514230 - 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(68-156)

Antitoxin

(14-97)


Sequences


Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T115900 WP_000227784.1 NZ_CP034399:c3508955-3508413 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T115900 NZ_CP034399:c3508955-3508413 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT115900 WP_001297137.1 NZ_CP034399:c3509250-3508939 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT115900 NZ_CP034399:c3509250-3508939 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 7BYY


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References