Detailed information of TA system TA115894

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2154401..2155235
• Replicon: chromosome
• Accession: NZ_CP034399
• Organism: Escherichia coli strain CRE1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A4S3WCD6
• Locus tag: EJC48_RS12330
• Protein ID: WP_021566610.1
• Coordinates: 2154858..2155235 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B3Y195
• Locus tag: EJC48_RS12325
• Protein ID: WP_001285585.1
• Coordinates: 2154401..2154769 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJC48_RS26285	2151402..2151760	+	359	Protein_2002	hypothetical protein	-
EJC48_RS12295	2151839..2152072	+	234	WP_001119729.1	DUF905 family protein	-
EJC48_RS12305	2152172..2152990	+	819	WP_001234629.1	DUF945 domain-containing protein	-
EJC48_RS12310	2153072..2153551	+	480	WP_048265132.1	antirestriction protein	-
EJC48_RS12315	2153567..2154043	+	477	WP_001186726.1	RadC family protein	-
EJC48_RS12320	2154106..2154327	+	222	WP_000692323.1	DUF987 domain-containing protein	-
EJC48_RS12325	2154401..2154769	+	369	WP_001285585.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
EJC48_RS12330	2154858..2155235	+	378	WP_021566610.1	TA system toxin CbtA family protein	Toxin
EJC48_RS12335	2155232..2155720	+	489	WP_048237286.1	hypothetical protein	-
EJC48_RS12340	2155732..2155929	+	198	WP_000839269.1	DUF957 domain-containing protein	-
EJC48_RS12345	2156014..2156859	+	846	WP_021566612.1	DUF4942 domain-containing protein	-
EJC48_RS12355	2157651..2158835	+	1185	WP_001172876.1	sugar efflux transporter	-
EJC48_RS12360	2158946..2159869	+	924	WP_000535971.1	carboxylate/amino acid/amine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14058.02 Da
• Isoelectric Point: 7.8276

>T115894 WP_021566610.1 NZ_CP034399:2154858-2155235 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T115894 NZ_CP034399:2154858-2155235 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.48 Da
• Isoelectric Point: 6.3139

>AT115894 WP_001285585.1 NZ_CP034399:2154401-2154769 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT115894 NZ_CP034399:2154401-2154769 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A4S3WCD6

Antitoxin

• AlphaFold DB: A0A7U9LW60