Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1290806..1291460 Replicon chromosome
Accession NZ_CP034399
Organism Escherichia coli strain CRE1

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag EJC48_RS08050 Protein ID WP_000244772.1
Coordinates 1290806..1291213 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EJC48_RS08055 Protein ID WP_000354046.1
Coordinates 1291194..1291460 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJC48_RS08030 1286763..1288496 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
EJC48_RS08035 1288502..1289212 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EJC48_RS08040 1289237..1290133 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EJC48_RS08045 1290245..1290766 + 522 WP_001055874.1 flavodoxin FldB -
EJC48_RS08050 1290806..1291213 - 408 WP_000244772.1 protein YgfX Toxin
EJC48_RS08055 1291194..1291460 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EJC48_RS08060 1291703..1292683 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
EJC48_RS08065 1292760..1293419 - 660 WP_000250274.1 hemolysin III family protein -
EJC48_RS08070 1293583..1293894 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
EJC48_RS08075 1293939..1295372 + 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
EJC48_RS08080 1295429..1296172 - 744 WP_000951940.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T115892 WP_000244772.1 NZ_CP034399:c1291213-1290806 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T115892 NZ_CP034399:c1291213-1290806 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT115892 WP_000354046.1 NZ_CP034399:c1291460-1291194 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT115892 NZ_CP034399:c1291460-1291194 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References