Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 831049..831674 Replicon chromosome
Accession NZ_CP034399
Organism Escherichia coli strain CRE1

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag EJC48_RS05825 Protein ID WP_000911329.1
Coordinates 831049..831447 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag EJC48_RS05830 Protein ID WP_000450524.1
Coordinates 831447..831674 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJC48_RS05805 826927..827127 + 201 WP_000383836.1 YpfN family protein -
EJC48_RS05810 827237..827935 - 699 WP_000679812.1 esterase -
EJC48_RS05815 828009..830024 - 2016 WP_000829332.1 tRNA cytosine(34) acetyltransferase TmcA -
EJC48_RS05820 830039..830902 - 864 WP_001267507.1 neutral zinc metallopeptidase -
EJC48_RS05825 831049..831447 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
EJC48_RS05830 831447..831674 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
EJC48_RS05835 831830..832543 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
EJC48_RS05840 832756..833790 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
EJC48_RS05845 833807..834685 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
EJC48_RS05850 834831..835403 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
EJC48_RS05855 835403..835873 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T115890 WP_000911329.1 NZ_CP034399:c831447-831049 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T115890 NZ_CP034399:c831447-831049 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAACATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT115890 WP_000450524.1 NZ_CP034399:c831674-831447 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT115890 NZ_CP034399:c831674-831447 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References