Detailed information of TA system TA115888

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 355137..355972
• Replicon: chromosome
• Accession: NZ_CP034399
• Organism: Escherichia coli strain CRE1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: E1IWA7
• Locus tag: EJC48_RS03600
• Protein ID: WP_000854751.1
• Coordinates: 355595..355972 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: EJC48_RS03595
• Protein ID: WP_001278232.1
• Coordinates: 355137..355505 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJC48_RS03560	351973..352428	+	456	WP_000581504.1	hypothetical protein	-
EJC48_RS03565	352507..352740	+	234	WP_001119729.1	DUF905 family protein	-
EJC48_RS03575	352840..353658	+	819	WP_001234630.1	DUF945 domain-containing protein	-
EJC48_RS03580	353713..354198	+	486	WP_000849582.1	antirestriction protein	-
EJC48_RS03585	354214..354690	+	477	WP_001186726.1	RadC family protein	-
EJC48_RS03590	354753..354974	+	222	WP_000692323.1	DUF987 domain-containing protein	-
EJC48_RS03595	355137..355505	+	369	WP_001278232.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
EJC48_RS03600	355595..355972	+	378	WP_000854751.1	TA system toxin CbtA family protein	Toxin
EJC48_RS03605	355969..356172	+	204	WP_000761704.1	hypothetical protein	-
EJC48_RS03610	356185..356298	+	114	WP_001161660.1	DUF957 domain-containing protein	-
EJC48_RS03615	356587..356715	-	129	Protein_344	transposase domain-containing protein	-
EJC48_RS03620	356787..356969	+	183	WP_001016348.1	hypothetical protein	-
EJC48_RS03625	357070..357399	-	330	WP_000450409.1	DUF496 family protein	-
EJC48_RS03630	357571..358629	-	1059	WP_001200910.1	FUSC family protein	-
EJC48_RS03635	358827..359300	-	474	WP_001105389.1	DNA gyrase inhibitor SbmC	-
EJC48_RS03640	359419..360585	-	1167	WP_001296209.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14050.04 Da
• Isoelectric Point: 8.2905

>T115888 WP_000854751.1 NZ_CP034399:355595-355972 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T115888 NZ_CP034399:355595-355972 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCCCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT115888 WP_001278232.1 NZ_CP034399:355137-355505 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT115888 NZ_CP034399:355137-355505 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: E1IWA7

Antitoxin

• AlphaFold DB: E3PJ72