Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 72513..73090 Replicon chromosome
Accession NZ_CP034395
Organism Herbaspirillum seropedicae strain AU13965

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag EJD96_RS00595 Protein ID WP_139969348.1
Coordinates 72513..72878 (-) Length 122 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag EJD96_RS00600 Protein ID WP_139972671.1
Coordinates 72872..73090 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJD96_RS00565 68129..68380 + 252 WP_139969343.1 ferrous iron transport protein A -
EJD96_RS00570 68377..70203 + 1827 WP_139969344.1 ferrous iron transporter B -
EJD96_RS00575 70200..70487 + 288 WP_139969345.1 hypothetical protein -
EJD96_RS00580 70507..71712 - 1206 WP_013232137.1 threonine ammonia-lyase -
EJD96_RS00585 71751..71999 - 249 WP_139969346.1 hypothetical protein -
EJD96_RS00590 72058..72414 + 357 WP_139969347.1 hypothetical protein -
EJD96_RS00595 72513..72878 - 366 WP_139969348.1 type II toxin-antitoxin system VapC family toxin Toxin
EJD96_RS00600 72872..73090 - 219 WP_139972671.1 ribbon-helix-helix protein, CopG family Antitoxin
EJD96_RS00605 73233..73805 + 573 WP_139969349.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
EJD96_RS00610 73824..74690 - 867 WP_013232140.1 ABC transporter permease -
EJD96_RS00615 74706..75578 - 873 WP_139969350.1 ABC transporter ATP-binding protein -
EJD96_RS00620 75614..76648 - 1035 WP_139969351.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-106)

Antitoxin

Ribbon-helix-helix protein, CopG

(6-41)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13817.01 Da        Isoelectric Point: 7.2024

>T115861 WP_139969348.1 NZ_CP034395:c72878-72513 [Herbaspirillum seropedicae]
MVKALFDTNILLDYLNGIAAAKKELARYEYRAISIVTWMEVMVGASAENEEALRSWLSSFHVINLDSALAERAVRIRQQW
RIRLPDAIVWASAMEHSLLLVSRNTKDFPVDEPGVRVPYKV

Download         Length: 366 bp

>T115861 NZ_CP034395:c72878-72513 [Herbaspirillum seropedicae]
ATGGTAAAAGCACTCTTCGACACCAATATCCTGCTGGATTATCTCAACGGCATAGCGGCTGCGAAAAAGGAACTGGCACG
CTATGAGTATCGCGCGATCAGCATCGTCACATGGATGGAAGTCATGGTGGGAGCCTCGGCGGAAAACGAGGAAGCCTTGC
GCTCCTGGCTCTCCTCGTTCCATGTGATCAATCTGGACAGCGCACTGGCAGAACGTGCGGTGCGCATCCGACAGCAATGG
CGGATACGATTACCAGATGCCATTGTCTGGGCGTCCGCCATGGAACATTCTTTATTGCTGGTATCGCGCAATACCAAGGA
TTTTCCGGTAGACGAGCCAGGCGTACGCGTTCCCTACAAGGTCTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8358.66 Da        Isoelectric Point: 8.5906

>AT115861 WP_139972671.1 NZ_CP034395:c73090-72872 [Herbaspirillum seropedicae]
MSRILVDIPESQLADLTQLVESQQRPRAVIIREAIAAYITQHKLEHDAEVFGIWKKKKVDGLAYQRALRSEW

Download         Length: 219 bp

>AT115861 NZ_CP034395:c73090-72872 [Herbaspirillum seropedicae]
ATGAGCCGGATCCTCGTTGACATTCCCGAGTCCCAATTGGCCGACCTGACCCAGCTTGTGGAAAGCCAGCAGCGTCCACG
TGCCGTCATCATACGTGAAGCGATTGCGGCCTACATTACGCAGCATAAGCTGGAGCATGATGCCGAGGTATTCGGCATCT
GGAAAAAGAAGAAGGTCGATGGATTGGCCTACCAAAGAGCGCTGCGTTCCGAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References