Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4563987..4564484 Replicon chromosome
Accession NZ_CP034389
Organism Escherichia coli strain RS571

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EJH97_RS22490 Protein ID WP_001173343.1
Coordinates 4563987..4564160 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag EJH97_RS22495 Protein ID WP_000398619.1
Coordinates 4564188..4564484 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJH97_RS22480 4560657..4562114 + 1458 WP_022646457.1 dipeptide/tripeptide permease DtpC -
EJH97_RS22485 4562351..4563868 + 1518 WP_022646456.1 lysine--tRNA ligase -
EJH97_RS22490 4563987..4564160 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EJH97_RS22495 4564188..4564484 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EJH97_RS22500 4564713..4564985 - 273 WP_000405647.1 N-acetyltransferase -
EJH97_RS22505 4564997..4565227 - 231 WP_128897029.1 (4Fe-4S)-binding protein -
EJH97_RS22510 4565408..4567039 + 1632 WP_022646455.1 sensor histidine kinase -
EJH97_RS22515 4567036..4567755 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4541226..4564484 23258


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T115858 WP_001173343.1 NZ_CP034389:c4564160-4563987 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T115858 NZ_CP034389:c4564160-4563987 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGCGCATTCCTGGTCGGAATAACCTGGCCAATGAGTTTACCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT115858 WP_000398619.1 NZ_CP034389:c4564484-4564188 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT115858 NZ_CP034389:c4564484-4564188 [Escherichia coli]
ATGGAAGGTAAAAATAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References