Detailed information of TA system TA115857

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4551928..4552763
• Replicon: chromosome
• Accession: NZ_CP034389
• Organism: Escherichia coli strain RS571

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q707F1
• Locus tag: EJH97_RS22440
• Protein ID: WP_001094445.1
• Coordinates: 4552386..4552763 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: EJH97_RS22435
• Protein ID: WP_024174331.1
• Coordinates: 4551928..4552296 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJH97_RS22415	4549677..4550495	+	819	WP_021548586.1	DUF945 domain-containing protein	-
EJH97_RS22420	4550587..4551072	+	486	WP_021538211.1	antirestriction protein	-
EJH97_RS22425	4551088..4551564	+	477	WP_001384029.1	RadC family protein	-
EJH97_RS22430	4551633..4551854	+	222	WP_000692346.1	DUF987 domain-containing protein	-
EJH97_RS22435	4551928..4552296	+	369	WP_024174331.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EJH97_RS22440	4552386..4552763	+	378	WP_001094445.1	TA system toxin CbtA family protein	Toxin
EJH97_RS22445	4552760..4553248	+	489	WP_000761717.1	hypothetical protein	-
EJH97_RS22450	4553260..4553457	+	198	WP_000838536.1	DUF957 domain-containing protein	-
EJH97_RS22455	4553542..4554384	+	843	WP_001280499.1	DUF4942 domain-containing protein	-
EJH97_RS22460	4555133..4556671	+	1539	WP_023908864.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4541226..4564484	23258

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14194.10 Da
• Isoelectric Point: 6.8518

>T115857 WP_001094445.1 NZ_CP034389:4552386-4552763 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRMVNNITQGKHPEAQQ

Nucleotide

• Length: 378 bp

>T115857 NZ_CP034389:4552386-4552763 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTACGAACTGACCAGCCAGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAATGGTAAATAACATTACCCAGGGTAAGCATCCGGAGGCGCAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.42 Da
• Isoelectric Point: 6.7386

>AT115857 WP_024174331.1 NZ_CP034389:4551928-4552296 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT115857 NZ_CP034389:4551928-4552296 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAACGTAA

Structures

Toxin

• AlphaFold DB: A0A454A7U6