Detailed information of TA system TA115856

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4390335..4390930
• Replicon: chromosome
• Accession: NZ_CP034389
• Organism: Escherichia coli strain RS571

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: EJH97_RS21615
• Protein ID: WP_000239581.1
• Coordinates: 4390335..4390685 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: EJH97_RS21620
• Protein ID: WP_001223213.1
• Coordinates: 4390679..4390930 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJH97_RS21595	4385789..4386811	-	1023	WP_001298067.1	ABC transporter permease	-
EJH97_RS21600	4386825..4388327	-	1503	WP_022646474.1	sugar ABC transporter ATP-binding protein	-
EJH97_RS21605	4388460..4389416	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
EJH97_RS21610	4389726..4390256	+	531	WP_000055075.1	inorganic diphosphatase	-
EJH97_RS21615	4390335..4390685	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
EJH97_RS21620	4390679..4390930	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
EJH97_RS21625	4391142..4391483	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
EJH97_RS21630	4391486..4395265	-	3780	WP_022646473.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T115856 WP_000239581.1 NZ_CP034389:c4390685-4390335 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T115856 NZ_CP034389:c4390685-4390335 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT115856 WP_001223213.1 NZ_CP034389:c4390930-4390679 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT115856 NZ_CP034389:c4390930-4390679 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7