Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4138267..4138817 Replicon chromosome
Accession NZ_CP034389
Organism Escherichia coli strain RS571

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag EJH97_RS20255 Protein ID WP_001160966.1
Coordinates 4138267..4138581 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag EJH97_RS20260 Protein ID WP_000125568.1
Coordinates 4138584..4138817 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJH97_RS20230 4133942..4135228 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
EJH97_RS20235 4135228..4136217 + 990 WP_000241224.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
EJH97_RS20240 4136214..4137035 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
EJH97_RS20245 4137038..4137415 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
EJH97_RS20250 4137422..4138270 + 849 WP_000257195.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
EJH97_RS20255 4138267..4138581 - 315 WP_001160966.1 CcdB family protein Toxin
EJH97_RS20260 4138584..4138817 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
EJH97_RS20265 4138903..4139382 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
EJH97_RS20270 4139574..4141436 - 1863 WP_022645165.1 glutathione-regulated potassium-efflux system protein KefC -
EJH97_RS20275 4141429..4141959 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
EJH97_RS20280 4142067..4143398 - 1332 WP_001183201.1 MFS transporter -
EJH97_RS20285 4143456..4143743 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T115853 WP_001160966.1 NZ_CP034389:c4138581-4138267 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T115853 NZ_CP034389:c4138581-4138267 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAATC
CAATCCTTTTACTGGTAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCACACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT115853 WP_000125568.1 NZ_CP034389:c4138817-4138584 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT115853 NZ_CP034389:c4138817-4138584 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References