Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3703162..3703780 Replicon chromosome
Accession NZ_CP034389
Organism Escherichia coli strain RS571

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EJH97_RS18195 Protein ID WP_001291435.1
Coordinates 3703562..3703780 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EJH97_RS18190 Protein ID WP_000344800.1
Coordinates 3703162..3703536 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJH97_RS18180 3698251..3699444 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
EJH97_RS18185 3699467..3702616 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
EJH97_RS18190 3703162..3703536 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EJH97_RS18195 3703562..3703780 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
EJH97_RS18200 3703952..3704503 + 552 WP_000102574.1 maltose O-acetyltransferase -
EJH97_RS18205 3704619..3705089 + 471 WP_022645280.1 YlaC family protein -
EJH97_RS18210 3705253..3706803 + 1551 WP_022645279.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EJH97_RS18215 3706845..3707198 - 354 WP_000878140.1 DUF1428 domain-containing protein -
EJH97_RS18225 3707577..3707888 + 312 WP_000409911.1 MGMT family protein -
EJH97_RS18230 3707919..3708491 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T115851 WP_001291435.1 NZ_CP034389:3703562-3703780 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T115851 NZ_CP034389:3703562-3703780 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACTATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT115851 WP_000344800.1 NZ_CP034389:3703162-3703536 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT115851 NZ_CP034389:3703162-3703536 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References