Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 74290..74849 Replicon plasmid pO157
Accession NZ_CP034385
Organism Escherichia coli O157:H7 strain FRIK804

Toxin (Protein)


Gene name relE Uniprot ID O82928
Locus tag EJB16_RS27825 Protein ID WP_000421248.1
Coordinates 74574..74849 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag EJB16_RS27820 Protein ID WP_001178089.1
Coordinates 74290..74574 (+) Length 95 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJB16_RS27780 69482..70042 + 561 WP_000840472.1 fertility inhibition protein FinO -
EJB16_RS27785 70175..70387 + 213 WP_001302189.1 ANR family transcriptional regulator -
EJB16_RS27790 70632..71093 + 462 WP_001233853.1 thermonuclease family protein -
EJB16_RS27795 71139..71348 + 210 WP_001302200.1 hemolysin expression modulator Hha -
EJB16_RS27800 71386..71724 + 339 WP_000766796.1 hypothetical protein -
EJB16_RS27805 71964..72218 + 255 WP_000083831.1 replication regulatory protein RepA -
EJB16_RS27810 72454..72528 + 75 WP_001370046.1 RepA leader peptide Tap -
EJB16_RS27815 72521..73378 + 858 WP_000130945.1 incFII family plasmid replication initiator RepA -
EJB16_RS27820 74290..74574 + 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
EJB16_RS27825 74574..74849 + 276 WP_000421248.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EJB16_RS27830 74944..75150 + 207 WP_001105064.1 hypothetical protein -
EJB16_RS27835 75250..75465 - 216 Protein_79 transposase -
EJB16_RS27840 75509..76075 - 567 Protein_80 IS3-like element IS600 family transposase -
EJB16_RS27845 76075..76491 - 417 Protein_81 IS91 family transposase -
EJB16_RS27850 76491..76676 - 186 WP_001302179.1 hypothetical protein -
EJB16_RS27855 76853..79063 + 2211 WP_000592771.1 catalase/peroxidase KatP -
EJB16_RS27860 79107..79496 + 390 WP_001172748.1 cytochrome b562 family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP 1..92697 92697


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 92 a.a.        Molecular weight: 10735.42 Da        Isoelectric Point: 9.9324

>T115833 WP_000421248.1 NZ_CP034385:74574-74849 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T115833 NZ_CP034385:74574-74849 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT115833 WP_001178089.1 NZ_CP034385:74290-74574 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT115833 NZ_CP034385:74290-74574 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A2Z6FAZ0


Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References