Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 28681..29206 Replicon plasmid pO157
Accession NZ_CP034385
Organism Escherichia coli O157:H7 strain FRIK804

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag EJB16_RS27570 Protein ID WP_001159868.1
Coordinates 28901..29206 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag EJB16_RS27565 Protein ID WP_000813634.1
Coordinates 28681..28899 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJB16_RS27535 23964..24194 + 231 WP_010891288.1 hypothetical protein -
EJB16_RS27540 24315..25055 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
EJB16_RS27545 25340..26317 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
EJB16_RS27550 26725..26925 - 201 WP_000708307.1 hypothetical protein -
EJB16_RS27555 26922..27542 - 621 WP_001248529.1 hypothetical protein -
EJB16_RS27560 27539..28222 - 684 WP_010891291.1 DNA-binding protein -
EJB16_RS27565 28681..28899 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
EJB16_RS27570 28901..29206 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
EJB16_RS27575 29207..30013 + 807 WP_000016989.1 site-specific integrase -
EJB16_RS27590 31911..32249 + 339 WP_071525396.1 RepB family plasmid replication initiator protein -
EJB16_RS27595 32837..34003 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP 1..92697 92697


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T115832 WP_001159868.1 NZ_CP034385:28901-29206 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T115832 NZ_CP034385:28901-29206 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT115832 WP_000813634.1 NZ_CP034385:28681-28899 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT115832 NZ_CP034385:28681-28899 [Escherichia coli O157:H7]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References