Detailed information of TA system TA115831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 5359709..5360304
• Replicon: chromosome
• Accession: NZ_CP034384
• Organism: Escherichia coli O157:H7 strain FRIK804

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: EJB16_RS26635
• Protein ID: WP_000239579.1
• Coordinates: 5359954..5360304 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: EJB16_RS26630
• Protein ID: WP_001223210.1
• Coordinates: 5359709..5359960 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJB16_RS26620	5355374..5359153	+	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-
EJB16_RS26625	5359156..5359497	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
EJB16_RS26630	5359709..5359960	+	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
EJB16_RS26635	5359954..5360304	+	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
EJB16_RS26640	5360384..5360914	-	531	WP_000055075.1	inorganic diphosphatase	-
EJB16_RS26645	5361224..5362180	+	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
EJB16_RS26650	5362320..5363822	+	1503	WP_000205806.1	sugar ABC transporter ATP-binding protein	-
EJB16_RS26655	5363836..5364858	+	1023	WP_001301928.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T115831 WP_000239579.1 NZ_CP034384:5359954-5360304 [Escherichia coli O157:H7]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T115831 NZ_CP034384:5359954-5360304 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT115831 WP_001223210.1 NZ_CP034384:5359709-5359960 [Escherichia coli O157:H7]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT115831 NZ_CP034384:5359709-5359960 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8