115820

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3727678..3728261 Replicon chromosome
Accession NZ_CP034384
Organism Escherichia coli O157:H7 strain FRIK804

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag EJB16_RS18835 Protein ID WP_000254738.1
Coordinates 3727678..3728013 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag EJB16_RS18840 Protein ID WP_000581937.1
Coordinates 3728013..3728261 (-) Length 83 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJB16_RS18820 3723565..3724863 - 1299 WP_000036723.1 phosphopyruvate hydratase -
EJB16_RS18825 3724951..3726588 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
EJB16_RS18830 3726816..3727607 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
EJB16_RS18835 3727678..3728013 - 336 WP_000254738.1 endoribonuclease MazF Toxin
EJB16_RS18840 3728013..3728261 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
EJB16_RS18845 3728339..3730573 - 2235 WP_000226815.1 GTP pyrophosphokinase -
EJB16_RS18850 3730621..3731922 - 1302 WP_000046824.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(10-109)

Antitoxin

(7-51)


Sequences


Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T115820 WP_000254738.1 NZ_CP034384:c3728013-3727678 [Escherichia coli O157:H7]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T115820 NZ_CP034384:c3728013-3727678 [Escherichia coli O157:H7]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT115820 WP_000581937.1 NZ_CP034384:c3728261-3728013 [Escherichia coli O157:H7]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT115820 NZ_CP034384:c3728261-3728013 [Escherichia coli O157:H7]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF


Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LMB4

References