Detailed information of TA system TA115819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2312783..2313421
• Replicon: chromosome
• Accession: NZ_CP034384
• Organism: Escherichia coli O157:H7 strain FRIK804

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: EJB16_RS11600
• Protein ID: WP_000813794.1
• Coordinates: 2313245..2313421 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EJB16_RS11595
• Protein ID: WP_001270286.1
• Coordinates: 2312783..2313199 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJB16_RS11575	2307935..2308876	-	942	WP_001251320.1	ABC transporter permease	-
EJB16_RS11580	2308877..2309890	-	1014	WP_000220402.1	ABC transporter ATP-binding protein	-
EJB16_RS11585	2309908..2311053	-	1146	WP_000047432.1	ABC transporter substrate-binding protein	-
EJB16_RS11590	2311298..2312704	-	1407	WP_000760654.1	PLP-dependent aminotransferase family protein	-
EJB16_RS11595	2312783..2313199	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
EJB16_RS11600	2313245..2313421	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
EJB16_RS11605	2313643..2313873	+	231	WP_000494244.1	YncJ family protein	-
EJB16_RS11610	2313965..2315926	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
EJB16_RS11615	2315999..2316535	-	537	WP_000429145.1	DNA-binding transcriptional regulator SutR	-
EJB16_RS11620	2316588..2317799	+	1212	WP_071525382.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T115819 WP_000813794.1 NZ_CP034384:c2313421-2313245 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T115819 NZ_CP034384:c2313421-2313245 [Escherichia coli O157:H7]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT115819 WP_001270286.1 NZ_CP034384:c2313199-2312783 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT115819 NZ_CP034384:c2313199-2312783 [Escherichia coli O157:H7]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCAGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB