Detailed information of TA system TA115797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4735429..4736015
• Replicon: chromosome
• Accession: NZ_CP034369
• Organism: Pseudomonas aeruginosa strain SP4371

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: EJA98_RS22535
• Protein ID: WP_003120987.1
• Coordinates: 4735716..4736015 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: EJA98_RS22530
• Protein ID: WP_003448662.1
• Coordinates: 4735429..4735719 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJA98_RS22515	4731010..4732899	+	1890	WP_033989533.1	hypothetical protein	-
EJA98_RS22520	4732896..4734872	+	1977	WP_022579622.1	DEAD/DEAH box helicase	-
EJA98_RS22525	4735011..4735346	+	336	WP_022579623.1	hypothetical protein	-
EJA98_RS22530	4735429..4735719	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
EJA98_RS22535	4735716..4736015	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EJA98_RS22540	4736217..4737341	+	1125	WP_023093672.1	TcpQ domain-containing protein	-
EJA98_RS22545	4737341..4739050	+	1710	WP_023093673.1	PilN family type IVB pilus formation outer membrane protein	-
EJA98_RS22550	4739054..4740379	+	1326	WP_022579625.1	type 4b pilus protein PilO2	-
EJA98_RS22555	4740369..4740902	+	534	WP_004352829.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4706492..4795934	89442

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T115797 WP_003120987.1 NZ_CP034369:c4736015-4735716 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T115797 NZ_CP034369:c4736015-4735716 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT115797 WP_003448662.1 NZ_CP034369:c4735719-4735429 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT115797 NZ_CP034369:c4735719-4735429 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
CTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCTCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCGACATTGAAAACCATGCTGGCA
GTAATGCGAGCTTTGGGCGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73