Detailed information of TA system TA115784

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1199857..1200485
• Replicon: chromosome
• Accession: NZ_CP034363
• Organism: Pantoea sp. CCBC3-3-1

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: EHV07_RS05455
• Protein ID: WP_147195830.1
• Coordinates: 1199857..1200075 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: EHV07_RS05460
• Protein ID: WP_147195832.1
• Coordinates: 1200105..1200485 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHV07_RS05425	1195753..1196091	+	339	WP_007886947.1	P-II family nitrogen regulator	-
EHV07_RS05430	1196127..1197416	+	1290	WP_147195823.1	ammonium transporter AmtB	-
EHV07_RS05435	1197482..1198339	-	858	WP_147195825.1	acyl-CoA thioesterase II	-
EHV07_RS05440	1198550..1199107	+	558	WP_147195826.1	YbaY family lipoprotein	-
EHV07_RS05445	1199148..1199459	-	312	WP_168199671.1	MGMT family protein	-
EHV07_RS05455	1199857..1200075	-	219	WP_147195830.1	hemolysin expression modulator Hha	Toxin
EHV07_RS05460	1200105..1200485	-	381	WP_147195832.1	Hha toxicity modulator TomB	Antitoxin
EHV07_RS05465	1200629..1200982	-	354	WP_147195834.1	hypothetical protein	-
EHV07_RS05470	1201398..1202078	+	681	WP_147195835.1	ABC transporter ATP-binding protein	-
EHV07_RS05475	1202075..1202950	+	876	WP_147195836.1	metal ABC transporter permease	-
EHV07_RS05480	1202947..1203825	+	879	WP_147195837.1	zinc ABC transporter substrate-binding protein	-
EHV07_RS05485	1203883..1204023	-	141	WP_147195839.1	type B 50S ribosomal protein L36	-
EHV07_RS05490	1204033..1204284	-	252	WP_147195841.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	acrB / acrA	1179950..1310593	130643

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8626.07 Da
• Isoelectric Point: 9.4825

>T115784 WP_147195830.1 NZ_CP034363:c1200075-1199857 [Pantoea sp. CCBC3-3-1]
MTDKLLTKTDYLMRLRRCRSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKVPVAVWKFVR

Nucleotide

• Length: 219 bp

>T115784 NZ_CP034363:c1200075-1199857 [Pantoea sp. CCBC3-3-1]
ATGACAGATAAACTCCTGACTAAAACTGATTATCTGATGCGTTTAAGACGGTGTCGGTCTATCGACACCCTCGAACGTGT
AATCGAGAAAAATAAATACGAATTATCCGATAATGAACTGGCAGTGTTCTATTCCGCTGCTGACCATCGTCTTGCTGAAC
TGACGATGAATAAGCTTTACGACAAAGTACCGGTTGCCGTTTGGAAATTTGTTCGCTAA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 14648.42 Da
• Isoelectric Point: 4.5911

>AT115784 WP_147195832.1 NZ_CP034363:c1200485-1200105 [Pantoea sp. CCBC3-3-1]
MDEYSPKRHDIAQLKFLCENLYDESLATLGDSHHGWVNDPTSAINLQLNDLIEHIAAFTMNYKIKHIEDSDLISQIDEYL
DDTFMLFSNYGINVQDLKRWQKSAKRLFNIFVEECAFVTAQASHSF

Nucleotide

• Length: 381 bp

>AT115784 NZ_CP034363:c1200485-1200105 [Pantoea sp. CCBC3-3-1]
ATGGACGAATACTCACCAAAAAGGCATGATATCGCCCAGCTTAAATTTTTGTGTGAAAACCTGTATGATGAAAGTCTTGC
CACCTTGGGTGACAGCCATCACGGTTGGGTTAACGATCCTACTTCAGCAATAAATTTGCAGCTTAACGACTTGATTGAGC
ATATTGCTGCTTTTACCATGAATTACAAAATCAAGCATATCGAAGATAGCGATCTGATTAGCCAGATAGATGAATATCTT
GACGATACGTTTATGCTTTTCAGTAATTACGGCATTAATGTTCAAGATCTCAAACGATGGCAAAAATCAGCGAAGCGGCT
GTTCAACATTTTTGTAGAGGAATGCGCTTTCGTCACTGCTCAGGCGAGCCATTCGTTTTAG