Detailed information of TA system TA115780

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 94105..94636
• Replicon: plasmid pPYO_TB
• Accession: NZ_CP034355
• Organism: Pseudomonas aeruginosa strain IMP-13

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A2K4Y311
• Locus tag: EIP87_RS34085
• Protein ID: WP_023100672.1
• Coordinates: 94105..94386 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: W2D9V6
• Locus tag: EIP87_RS34090
• Protein ID: WP_020303033.1
• Coordinates: 94373..94636 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EIP87_RS34035	89426..90109	+	684	WP_034018055.1	hypothetical protein	-
EIP87_RS34040	90118..90516	+	399	WP_176742248.1	hypothetical protein	-
EIP87_RS34045	90567..91043	+	477	WP_034026837.1	single-stranded DNA-binding protein	-
EIP87_RS34050	91117..91536	+	420	WP_057380713.1	hypothetical protein	-
EIP87_RS34055	91533..91781	+	249	WP_034017785.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
EIP87_RS34060	91778..92128	+	351	WP_023100676.1	type II toxin-antitoxin system PemK/MazF family toxin	-
EIP87_RS34065	92128..92850	+	723	WP_031634065.1	hypothetical protein	-
EIP87_RS34070	92899..93351	-	453	WP_023100674.1	hypothetical protein	-
EIP87_RS34080	93733..94068	-	336	WP_023100673.1	hypothetical protein	-
EIP87_RS34085	94105..94386	-	282	WP_023100672.1	type II toxin-antitoxin system YafQ family toxin	Toxin
EIP87_RS34090	94373..94636	-	264	WP_020303033.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
EIP87_RS34095	94960..95703	-	744	WP_023100671.1	hypothetical protein	-
EIP87_RS34100	95935..96426	-	492	WP_023100670.1	hypothetical protein	-
EIP87_RS34105	96426..97772	-	1347	WP_125728849.1	replicative DNA helicase	-
EIP87_RS34110	97959..98420	-	462	WP_033955307.1	hypothetical protein	-
EIP87_RS34115	98613..98930	-	318	WP_171942272.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..130306	130306

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-84)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10724.19 Da
• Isoelectric Point: 9.0277

>T115780 WP_023100672.1 NZ_CP034355:c94386-94105 [Pseudomonas aeruginosa]
MRTIEQTSRFKRDYKREAKGPHRQTLASDFVAIVTALANDQPLAEKHRDHALNGDWKDHRDCHIKPDLVLIYRKPDDAVL
QLVRLGSHSELGL

Nucleotide

• Length: 282 bp

>T115780 NZ_CP034355:c94386-94105 [Pseudomonas aeruginosa]
ATGCGGACGATTGAACAGACCAGCCGGTTCAAGCGCGATTACAAACGCGAAGCCAAAGGCCCGCATCGTCAGACGCTGGC
GAGCGATTTCGTCGCCATTGTCACAGCCCTTGCGAATGACCAGCCGCTGGCCGAGAAGCACCGCGACCACGCGCTCAATG
GCGACTGGAAAGATCACCGGGACTGCCACATCAAGCCTGACCTGGTGCTGATTTACCGCAAGCCGGATGACGCCGTGTTG
CAACTCGTGCGTCTCGGCTCGCATAGCGAGCTTGGCCTGTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9471.95 Da
• Isoelectric Point: 4.8096

>AT115780 WP_020303033.1 NZ_CP034355:c94636-94373 [Pseudomonas aeruginosa]
MSANAVVRARIDEHIKEEATVVLAAMGLTVSDAFRIMLTRVAREKALPFEPLIPNATTIEAMKEARRGGLKSFASVEDLM
ADLNADD

Nucleotide

• Length: 264 bp

>AT115780 NZ_CP034355:c94636-94373 [Pseudomonas aeruginosa]
ATGTCTGCCAATGCTGTAGTCCGTGCCCGTATCGACGAGCACATCAAGGAAGAGGCGACCGTCGTGCTGGCGGCGATGGG
GCTCACCGTGTCCGACGCCTTCCGCATCATGTTGACCCGCGTCGCCCGCGAGAAAGCCTTGCCGTTCGAGCCGCTGATTC
CGAACGCCACCACCATCGAGGCGATGAAGGAAGCGCGCCGTGGTGGCCTGAAGTCGTTTGCTTCCGTCGAGGACCTGATG
GCGGACCTGAATGCGGACGATTGA

Structures

Toxin

• AlphaFold DB: A0A2K4Y311

Antitoxin

• AlphaFold DB: A0A0W0N2R7