Detailed information of TA system TA115779

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpB-mazE/PRK09812-ChpS
• Location: 91533..92128
• Replicon: plasmid pPYO_TB
• Accession: NZ_CP034355
• Organism: Pseudomonas aeruginosa strain IMP-13

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: A0A2K4Y2Z0
• Locus tag: EIP87_RS34060
• Protein ID: WP_023100676.1
• Coordinates: 91778..92128 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: EIP87_RS34055
• Protein ID: WP_034017785.1
• Coordinates: 91533..91781 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EIP87_RS34020	86601..88676	+	2076	WP_034018058.1	DNA cytosine methyltransferase	-
EIP87_RS34025	88666..88896	+	231	WP_034018057.1	hypothetical protein	-
EIP87_RS34030	88893..89429	+	537	WP_034018056.1	hypothetical protein	-
EIP87_RS34035	89426..90109	+	684	WP_034018055.1	hypothetical protein	-
EIP87_RS34040	90118..90516	+	399	WP_176742248.1	hypothetical protein	-
EIP87_RS34045	90567..91043	+	477	WP_034026837.1	single-stranded DNA-binding protein	-
EIP87_RS34050	91117..91536	+	420	WP_057380713.1	hypothetical protein	-
EIP87_RS34055	91533..91781	+	249	WP_034017785.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EIP87_RS34060	91778..92128	+	351	WP_023100676.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EIP87_RS34065	92128..92850	+	723	WP_031634065.1	hypothetical protein	-
EIP87_RS34070	92899..93351	-	453	WP_023100674.1	hypothetical protein	-
EIP87_RS34080	93733..94068	-	336	WP_023100673.1	hypothetical protein	-
EIP87_RS34085	94105..94386	-	282	WP_023100672.1	type II toxin-antitoxin system YafQ family toxin	-
EIP87_RS34090	94373..94636	-	264	WP_020303033.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
EIP87_RS34095	94960..95703	-	744	WP_023100671.1	hypothetical protein	-
EIP87_RS34100	95935..96426	-	492	WP_023100670.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..130306	130306

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (11-112)

Antitoxin

• Antitoxin PemI-like (1-81)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12073.06 Da
• Isoelectric Point: 10.1005

>T115779 WP_023100676.1 NZ_CP034355:91778-92128 [Pseudomonas aeruginosa]
MSKRLQHQFDRGDIVSLDMGSASAQAACKALVLSPAAYNVLGLALAIPITEHDSSRYAGFAVAILLPGRTPAKGAALVNL
VRPVDLAARGAKLLGKAPQTTIDEALQRLQAVVGKD

Nucleotide

• Length: 351 bp

>T115779 NZ_CP034355:91778-92128 [Pseudomonas aeruginosa]
ATGAGCAAACGCCTGCAGCATCAGTTCGACCGCGGCGATATCGTCTCCTTGGACATGGGTAGCGCTTCAGCACAGGCGGC
CTGCAAAGCGCTCGTGCTTTCCCCTGCAGCGTACAACGTCCTGGGGCTCGCCCTAGCCATTCCTATCACCGAGCACGACA
GCAGCCGCTACGCCGGCTTTGCCGTCGCGATCCTCCTTCCAGGGCGCACGCCGGCGAAGGGGGCAGCCTTGGTCAACCTG
GTGCGGCCGGTGGATCTTGCTGCAAGAGGTGCAAAGCTGCTCGGGAAGGCGCCCCAGACGACCATAGATGAAGCCCTGCA
GCGCCTGCAGGCGGTTGTTGGGAAAGACTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8753.01 Da
• Isoelectric Point: 4.1093

>AT115779 WP_034017785.1 NZ_CP034355:91533-91781 [Pseudomonas aeruginosa]
MKTTIRAIGNSQGITIPKPLLAELGVSLGDPVDISFKDQTLVVTKVATEYDLSTLVAQCDLEAPEPPDIQCWRTATPVGN
EA

Nucleotide

• Length: 249 bp

>AT115779 NZ_CP034355:91533-91781 [Pseudomonas aeruginosa]
TTGAAAACGACTATCCGTGCGATTGGGAACTCTCAGGGCATCACGATACCCAAGCCGTTGCTGGCCGAGCTCGGCGTAAG
CCTTGGCGACCCTGTAGACATCTCCTTCAAAGACCAGACCTTGGTGGTGACCAAGGTCGCCACCGAATATGACCTCAGCA
CGCTAGTCGCCCAGTGTGACCTGGAGGCCCCGGAGCCGCCGGACATCCAATGCTGGAGAACAGCTACGCCGGTAGGAAAC
GAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A2K4Y2Z0