Detailed information of TA system TA115758

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 4963972..4964598
• Replicon: chromosome
• Accession: NZ_CP034346
• Organism: Paenibacillus lutimineralis strain MBLB1234

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A3Q9IBG5
• Locus tag: EI981_RS22065
• Protein ID: WP_068779893.1
• Coordinates: 4963972..4964322 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: EI981_RS22070
• Protein ID: WP_068779892.1
• Coordinates: 4964326..4964598 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EI981_RS22045	4959868..4960236	-	369	WP_127001914.1	DUF4190 domain-containing protein	-
EI981_RS22050	4960535..4961953	+	1419	WP_127001916.1	efflux RND transporter periplasmic adaptor subunit	-
EI981_RS22055	4961953..4962660	+	708	WP_127001918.1	ABC transporter ATP-binding protein	-
EI981_RS22060	4962657..4963829	+	1173	WP_127001920.1	ABC transporter permease	-
EI981_RS22065	4963972..4964322	-	351	WP_068779893.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EI981_RS22070	4964326..4964598	-	273	WP_068779892.1	ribbon-helix-helix protein, CopG family	Antitoxin
EI981_RS22075	4964851..4966035	-	1185	WP_127004889.1	alanine racemase	-
EI981_RS22080	4966249..4967382	-	1134	WP_127001923.1	outer membrane lipoprotein carrier protein LolA	-
EI981_RS22085	4968028..4969014	+	987	WP_127001925.1	tryptophan--tRNA ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12762.75 Da
• Isoelectric Point: 5.6126

>T115758 WP_068779893.1 NZ_CP034346:c4964322-4963972 [Paenibacillus lutimineralis]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVILLEQI
RTIDKQRLTDKITHLDDETMKKVNDALLISLGLIDF

Nucleotide

• Length: 351 bp

>T115758 NZ_CP034346:c4964322-4963972 [Paenibacillus lutimineralis]
TTGATCGTAAAGCGCGGTGACGTATTCTTTGCAGACCTTTCCCCGGTAGTTGGTTCTGAACAGGGAGGGGTAAGACCGGT
GCTAATCATCCAGAACGATATCGGCAATCGATTTAGCCCAACGGCGATCGTTGCTGCCATCACAGCTCAAATTCAAAAGG
CAAAGCTGCCGACTCACGTTGAAATTGATGCAGCGACGCACGGATTTGATCGTGATTCCGTCATTTTGCTGGAACAGATT
CGAACGATCGACAAACAAAGATTAACCGACAAAATCACACATTTAGACGATGAGACGATGAAGAAAGTGAATGATGCATT
GTTGATTAGTCTAGGTTTGATTGATTTTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10458.10 Da
• Isoelectric Point: 6.4710

>AT115758 WP_068779892.1 NZ_CP034346:c4964598-4964326 [Paenibacillus lutimineralis]
MHNTKRIMISLPDHLLQEVDGIVAMENSNRSELIRQAMKLYLNERKKRFIRESMQRGYMEMAKINLSMASEAFQAEEDAD
ITLDRLVSGV

Nucleotide

• Length: 273 bp

>AT115758 NZ_CP034346:c4964598-4964326 [Paenibacillus lutimineralis]
ATGCACAATACCAAACGAATCATGATCAGTTTACCGGATCATCTTTTACAGGAGGTAGATGGGATCGTGGCGATGGAGAA
TTCCAATCGCAGCGAACTGATTCGGCAAGCCATGAAGCTGTATCTGAACGAGCGAAAGAAGAGATTTATCCGCGAGTCGA
TGCAGCGGGGTTACATGGAAATGGCTAAGATTAACCTATCCATGGCCTCTGAAGCGTTCCAAGCAGAGGAAGATGCAGAC
ATCACTCTGGACCGCCTAGTAAGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A3Q9IBG5