Detailed information of TA system TA115731

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 2780030..2780646
• Replicon: chromosome
• Accession: NZ_CP034336
• Organism: Enterobacter asburiae strain CAV1043

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EI562_RS13945
• Protein ID: WP_023309752.1
• Coordinates: 2780275..2780646 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A808IJD0
• Locus tag: EI562_RS13940
• Protein ID: WP_023309753.1
• Coordinates: 2780030..2780272 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EI562_RS13915	2776007..2776606	+	600	WP_023309757.1	glucose-1-phosphatase	-
EI562_RS13920	2776600..2777481	+	882	WP_023309756.1	virulence factor BrkB family protein	-
EI562_RS13925	2777478..2777915	+	438	WP_008501836.1	D-tyrosyl-tRNA(Tyr) deacylase	-
EI562_RS13930	2777960..2778901	+	942	WP_023309755.1	fatty acid biosynthesis protein FabY	-
EI562_RS13935	2778910..2779830	-	921	WP_023309754.1	alpha/beta hydrolase	-
EI562_RS13940	2780030..2780272	+	243	WP_023309753.1	ribbon-helix-helix protein, CopG family	Antitoxin
EI562_RS13945	2780275..2780646	+	372	WP_023309752.1	type II toxin-antitoxin system VapC family toxin	Toxin
EI562_RS13950	2780686..2781615	-	930	WP_023309751.1	formate dehydrogenase accessory protein FdhE	-
EI562_RS13955	2781612..2782247	-	636	WP_023309750.1	formate dehydrogenase cytochrome b556 subunit	-
EI562_RS13960	2782244..2783146	-	903	WP_008501831.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-109)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13714.88 Da
• Isoelectric Point: 6.8710

>T115731 WP_023309752.1 NZ_CP034336:2780275-2780646 [Enterobacter asburiae]
MEHMAVFDTNILIDLFNNRVEAADAIDRTASHRAISLITWMEVMVGARKHGHEAKTATVMGVFEIIDITRDIAERSVILR
EKYGMKLPDAIILATAQSRNCPLISRNTKDFSGITGVVSPYQL

Nucleotide

• Length: 372 bp

>T115731 NZ_CP034336:2780275-2780646 [Enterobacter asburiae]
ATGGAACACATGGCGGTGTTCGATACCAATATACTCATTGATCTATTTAATAATCGCGTTGAGGCTGCGGATGCAATTGA
TCGCACAGCTTCGCATCGAGCCATTAGCCTGATCACATGGATGGAAGTCATGGTGGGTGCCCGCAAACATGGTCACGAAG
CGAAAACTGCGACAGTGATGGGCGTATTTGAGATTATTGATATCACCCGAGATATCGCTGAAAGAAGTGTAATTCTGCGC
GAGAAGTATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCTCAGAGCAGAAATTGTCCGTTGATTTCTCGAAA
CACGAAAGACTTTTCGGGCATTACTGGAGTCGTTTCACCTTACCAACTATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9429.56 Da
• Isoelectric Point: 4.2803

>AT115731 WP_023309753.1 NZ_CP034336:2780030-2780272 [Enterobacter asburiae]
MSLSADLDMGRILIDLSDDVIQRLDNLKQLRNRPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT115731 NZ_CP034336:2780030-2780272 [Enterobacter asburiae]
ATGAGTCTTTCCGCGGATCTTGATATGGGCAGAATTCTGATCGATTTGTCTGATGACGTAATTCAACGACTGGATAATCT
GAAGCAGCTGCGTAACCGGCCTCGTGCCGAATTGCTTCGGGAAGCCATTGAGCAATATCTCGACCAGCAGAGTTCATCGG
TTATCCGCGATGCGCTTGGCTTGTGGGGAAACCAGCAGGAAGATGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A808IJD0