Detailed information of TA system TA115728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): KacT-ataR/DUF1778(antitoxin)
• Location: 894994..895791
• Replicon: chromosome
• Accession: NZ_CP034336
• Organism: Enterobacter asburiae strain CAV1043

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: EI562_RS04840
• Protein ID: WP_173654903.1
• Coordinates: 894994..895494 (-)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: EI562_RS04845
• Protein ID: WP_023311079.1
• Coordinates: 895522..895791 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EI562_RS04810	890577..891266	+	690	WP_023311085.1	pyrimidine utilization protein B	-
EI562_RS04815	891278..891664	+	387	WP_010429508.1	pyrimidine utilization protein C	-
EI562_RS04820	891672..892472	+	801	WP_023311084.1	pyrimidine utilization protein D	-
EI562_RS04825	892482..893072	+	591	WP_023311083.1	malonic semialdehyde reductase	-
EI562_RS04830	893082..893576	+	495	WP_023311082.1	pyrimidine utilization flavin reductase protein F	-
EI562_RS04835	893598..894920	+	1323	WP_023311081.1	pyrimidine utilization transport protein G	-
EI562_RS04840	894994..895494	-	501	WP_173654903.1	GNAT family N-acetyltransferase	Toxin
EI562_RS04845	895522..895791	-	270	WP_023311079.1	DUF1778 domain-containing protein	Antitoxin
EI562_RS04850	895853..896770	-	918	WP_023311078.1	DMT family transporter	-
EI562_RS04855	896888..897058	-	171	WP_023311077.1	general stress protein	-
EI562_RS04860	897450..898046	+	597	WP_023311076.1	NAD(P)H:quinone oxidoreductase	-
EI562_RS04865	898067..898294	+	228	WP_008499832.1	YccJ family protein	-
EI562_RS04870	898328..899569	-	1242	WP_023311075.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
EI562_RS04875	899722..900165	+	444	WP_023311074.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (2-88)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18955.64 Da
• Isoelectric Point: 7.9145

>T115728 WP_173654903.1 NZ_CP034336:c895494-894994 [Enterobacter asburiae]
MLSEGTDYDFEDFDCGEPSLNAFLAEHLVRQHNGRILRAYLLKERDRPRVLGYYTLSGSCFERAMLPSKTQQRRIPYINV
PSVTLGRLAVDKTLQGNEWGTTLVAHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKQGFIPLTGENSHSLFFPTKSIE
RLFEQE

Nucleotide

• Length: 501 bp

>T115728 NZ_CP034336:c895494-894994 [Enterobacter asburiae]
ATGTTATCAGAGGGAACCGATTATGACTTCGAGGATTTTGATTGTGGCGAACCCTCGCTAAATGCCTTCCTTGCTGAACA
TCTGGTGCGTCAGCACAATGGGCGTATTTTACGAGCCTACCTGCTTAAAGAGCGCGATCGTCCGCGTGTCCTGGGTTATT
ACACGTTATCGGGAAGCTGTTTTGAAAGAGCGATGCTTCCTTCTAAGACTCAGCAGCGCCGGATCCCTTATATCAACGTA
CCCAGCGTGACGCTGGGACGTCTGGCTGTAGATAAAACACTACAGGGAAACGAGTGGGGAACAACGCTCGTCGCGCATGC
TATGCGCGTTGTCTACCTGGCTTCACAGGCTGTGGGGGTACACGGAATATTTGTTGATGCCCTTAACGAGCGGGCAAAAC
GATTTTATCTGAAGCAGGGCTTTATCCCGCTGACAGGTGAAAACAGCCATTCTTTGTTCTTCCCTACAAAATCTATCGAA
CGGCTGTTTGAGCAAGAGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10249.72 Da
• Isoelectric Point: 5.8836

>AT115728 WP_023311079.1 NZ_CP034336:c895791-895522 [Enterobacter asburiae]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWTAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT115728 NZ_CP034336:c895791-895522 [Enterobacter asburiae]
ATGCCAGCACTTAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCGGCGAT
GACGAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCAGAGGTCATTGAGCAGCACCGTCGTC
TCGTATTAAACGAAGCATCATGGACTGCGGTGATGGATGCCATCGACAATCCGCCTGAGCCAAATGAACGCCTGAAACGA
GCAGCAAAACGGCTTCGAAACATGGAGTGA