Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 54731..55374 Replicon plasmid pK033_1
Accession NZ_CP034322
Organism Klebsiella pneumoniae strain 33

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag EJB01_RS26675 Protein ID WP_000754566.1
Coordinates 54731..55147 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag EJB01_RS26680 Protein ID WP_001261276.1
Coordinates 55144..55374 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJB01_RS26660 49779..51887 - 2109 WP_016338368.1 peptidase domain-containing ABC transporter -
EJB01_RS26665 51877..53154 - 1278 WP_016338369.1 HlyD family secretion protein -
EJB01_RS26670 53813..54690 + 878 Protein_55 restriction endonuclease -
EJB01_RS26675 54731..55147 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
EJB01_RS26680 55144..55374 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
EJB01_RS26685 55331..55804 + 474 Protein_58 hypothetical protein -
EJB01_RS26695 55965..56909 + 945 WP_011977810.1 hypothetical protein -
EJB01_RS26700 56946..57338 + 393 WP_011977811.1 hypothetical protein -
EJB01_RS26705 57396..57917 + 522 WP_013214008.1 hypothetical protein -
EJB01_RS26710 57963..58166 + 204 WP_011977813.1 hypothetical protein -
EJB01_RS26715 58196..59200 + 1005 WP_011977814.1 hypothetical protein -
EJB01_RS26725 59384..60163 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / aac(3)-IId / blaTEM-1B / blaCTX-M-15 / dfrA30 - 1..150094 150094


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T115725 WP_000754566.1 NZ_CP034322:c55147-54731 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T115725 NZ_CP034322:c55147-54731 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT115725 WP_001261276.1 NZ_CP034322:c55374-55144 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT115725 NZ_CP034322:c55374-55144 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References