Detailed information of TA system TA115697

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 2058435..2058951
• Replicon: chromosome
• Accession: NZ_CP034298
• Organism: Vibrio parahaemolyticus strain 20160303005-1

Toxin (Protein)

• Gene name: relB
• Uniprot ID: U2ZZV8
• Locus tag: EHC69_RS10025
• Protein ID: WP_005464459.1
• Coordinates: 2058435..2058704 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A7W4CB60
• Locus tag: EHC69_RS10030
• Protein ID: WP_005483180.1
• Coordinates: 2058697..2058951 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHC69_RS09990	2054597..2055043	-	447	Protein_1838	GNAT family N-acetyltransferase	-
EHC69_RS09995	2055222..2055632	-	411	WP_024701711.1	hypothetical protein	-
EHC69_RS10000	2055713..2056603	-	891	WP_005485669.1	hypothetical protein	-
EHC69_RS10005	2056790..2056845	-	56	Protein_1841	DUF3265 domain-containing protein	-
EHC69_RS10015	2057113..2057913	-	801	WP_159408403.1	IS3 family transposase	-
EHC69_RS10020	2057997..2058218	-	222	Protein_1843	transposase	-
EHC69_RS10025	2058435..2058704	-	270	WP_005464459.1	Txe/YoeB family addiction module toxin	Toxin
EHC69_RS10030	2058697..2058951	-	255	WP_005483180.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
EHC69_RS10035	2059054..2059173	-	120	WP_078581129.1	DUF3265 domain-containing protein	-
EHC69_RS10040	2059160..2059534	-	375	WP_025787405.1	hypothetical protein	-
EHC69_RS10050	2060183..2060578	-	396	WP_017449623.1	ACT domain-containing protein	-
EHC69_RS10055	2060732..2061322	-	591	WP_045588482.1	hypothetical protein	-
EHC69_RS10060	2061471..2061932	-	462	WP_025787408.1	RidA family protein	-
EHC69_RS10065	2062075..2063025	-	951	WP_031815789.1	DUF4062 domain-containing protein	-
EHC69_RS10070	2063172..2063681	-	510	WP_000100093.1	ClbS/DfsB family four-helix bundle protein	-
EHC69_RS10075	2063709..2063801	-	93	WP_078581101.1	DUF3265 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2052855..2139950	87095
	-	flank	IS/Tn	-	-	2053897..2054283	386
	-	inside	Integron	-	-	2054735..2138239	83504

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (10-89)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10614.96 Da
• Isoelectric Point: 7.9816

>T115697 WP_005464459.1 NZ_CP034298:c2058704-2058435 [Vibrio parahaemolyticus]
MSSSQRLLSWTDDAWDDYLYWQTQDKKTLKRINKLINDVKRSPFEGIGKPEPLKENLSGFWSRRIDDTNRLVYAVDDQAI
TIISCRYHY

Nucleotide

• Length: 270 bp

>T115697 NZ_CP034298:c2058704-2058435 [Vibrio parahaemolyticus]
ATGAGTAGTAGTCAACGTTTATTATCGTGGACTGATGATGCTTGGGATGACTACCTGTATTGGCAAACTCAAGACAAGAA
AACACTCAAGCGCATCAATAAACTCATCAATGATGTTAAGCGCTCTCCATTTGAGGGCATTGGTAAACCAGAGCCGTTAA
AAGAGAACTTATCTGGTTTTTGGTCTCGTCGTATTGATGATACTAATAGGCTTGTTTACGCAGTCGATGATCAAGCGATA
ACGATAATTTCATGTCGTTACCACTACTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9481.66 Da
• Isoelectric Point: 5.0623

>AT115697 WP_005483180.1 NZ_CP034298:c2058951-2058697 [Vibrio parahaemolyticus]
MRIVSFTEARNGLKAVLDGVVNDADTTVITRRDSEDAVVMSLDYYNSLMETVHLLRSPQNVEHLNRSIAQYRAGKTTARE
LIDE

Nucleotide

• Length: 255 bp

>AT115697 NZ_CP034298:c2058951-2058697 [Vibrio parahaemolyticus]
ATGAGAATCGTATCTTTTACTGAAGCTAGAAATGGTCTCAAAGCTGTTTTAGACGGTGTAGTTAATGATGCTGATACAAC
AGTTATTACACGCCGTGATTCTGAGGATGCAGTGGTTATGTCTTTAGACTACTACAATAGCCTTATGGAGACTGTTCATT
TACTACGCTCTCCTCAAAACGTTGAACACCTAAACCGTTCGATAGCACAGTACCGCGCTGGTAAAACAACAGCACGAGAG
TTAATTGATGAGTAG

Structures

Toxin

• AlphaFold DB: U2ZZV8

Antitoxin

• AlphaFold DB: A0A7W4CB60