Detailed information of TA system TA115696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 34218..34786
• Replicon: plasmid pVPGD2014-1
• Accession: NZ_CP034296
• Organism: Vibrio parahaemolyticus strain 20140829008-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1Y1BA95
• Locus tag: EHC66_RS25765
• Protein ID: WP_023624650.1
• Coordinates: 34445..34786 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: EHC66_RS25760
• Protein ID: WP_017449238.1
• Coordinates: 34218..34448 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHC66_RS25735	29564..30595	-	1032	WP_159408187.1	Ig-like domain-containing protein	-
EHC66_RS25740	31156..31530	-	375	WP_127914005.1	hypothetical protein	-
EHC66_RS25745	31840..32061	+	222	WP_023624649.1	hypothetical protein	-
EHC66_RS25750	32664..33047	+	384	Protein_42	recombinase family protein	-
EHC66_RS25755	33042..33767	-	726	WP_017449237.1	site-specific integrase	-
EHC66_RS25760	34218..34448	+	231	WP_017449238.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EHC66_RS25765	34445..34786	+	342	WP_023624650.1	endoribonuclease MazF	Toxin
EHC66_RS25770	35089..35664	-	576	WP_017449240.1	hypothetical protein	-
EHC66_RS25775	35664..36395	-	732	WP_025789518.1	AHH domain-containing protein	-
EHC66_RS25785	37638..38726	-	1089	WP_086370877.1	replication initiation protein	-
EHC66_RS25790	39060..39698	-	639	Protein_49	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..106440	106440

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-107)

Antitoxin

• Antitoxin PemI-like (1-75)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12159.65 Da
• Isoelectric Point: 5.8124

>T115696 WP_023624650.1 NZ_CP034296:34445-34786 [Vibrio parahaemolyticus]
MTYAVPEKGDVVVMSFNPQSGHEQAGDSRPGLVISPKEFNQATGFALVCPITNQSKGYPFEVEITGSQKTTGVVLADQVK
SLDWRARRARVVDSVSNETLGTVRDYVTAIANG

Nucleotide

• Length: 342 bp

>T115696 NZ_CP034296:34445-34786 [Vibrio parahaemolyticus]
ATGACGTATGCAGTACCAGAGAAAGGCGATGTAGTAGTAATGAGTTTTAATCCGCAAAGCGGACACGAACAAGCGGGAGA
CAGTCGGCCTGGGCTGGTCATTTCCCCGAAAGAGTTTAACCAAGCAACTGGCTTTGCTCTGGTATGCCCCATAACCAACC
AGTCAAAAGGGTATCCGTTTGAAGTTGAAATAACCGGAAGCCAAAAAACAACGGGAGTGGTGCTGGCTGACCAAGTGAAG
TCCTTGGATTGGCGCGCGCGTCGCGCTCGTGTGGTAGACTCCGTTTCAAACGAAACACTGGGGACTGTGCGGGATTATGT
CACAGCGATTGCTAATGGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8499.87 Da
• Isoelectric Point: 6.4604

>AT115696 WP_017449238.1 NZ_CP034296:34218-34448 [Vibrio parahaemolyticus]
MSQTIAKWGHSAAVRIPSKVLKNMDMAVGDKVEVKVFNRQIIIEPAKPTLAQLLSQITDENRHDEVITETMGNELL

Nucleotide

• Length: 231 bp

>AT115696 NZ_CP034296:34218-34448 [Vibrio parahaemolyticus]
ATGTCACAAACAATAGCTAAGTGGGGCCACAGCGCAGCTGTGCGTATACCTTCTAAGGTATTGAAAAATATGGATATGGC
GGTGGGTGACAAAGTGGAGGTTAAAGTGTTCAATCGACAAATTATCATTGAACCTGCAAAACCAACACTCGCCCAGCTTT
TGAGTCAGATCACTGATGAAAACCGCCATGATGAAGTAATCACCGAAACTATGGGTAATGAATTGTTATGA

Structures

Toxin

• AlphaFold DB: A0A1Y1BA95