Detailed information of TA system TA115683

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 13934..14502
• Replicon: plasmid pVPHB2014-1
• Accession: NZ_CP034287
• Organism: Vibrio parahaemolyticus strain 20140624012-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1Y1BA95
• Locus tag: EHC70_RS25135
• Protein ID: WP_023624650.1
• Coordinates: 13934..14275 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: EHC70_RS25140
• Protein ID: WP_017449238.1
• Coordinates: 14272..14502 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHC70_RS25110	9022..9660	+	639	Protein_9	IS5 family transposase	-
EHC70_RS25115	9994..11082	+	1089	WP_086370877.1	replication initiation protein	-
EHC70_RS25125	12325..13056	+	732	WP_025789518.1	AHH domain-containing protein	-
EHC70_RS25130	13056..13631	+	576	WP_017449240.1	hypothetical protein	-
EHC70_RS25135	13934..14275	-	342	WP_023624650.1	endoribonuclease MazF	Toxin
EHC70_RS25140	14272..14502	-	231	WP_017449238.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EHC70_RS25145	14953..15678	+	726	WP_017449237.1	site-specific integrase	-
EHC70_RS25150	15673..16056	-	384	Protein_16	recombinase family protein	-
EHC70_RS25155	16659..16880	-	222	WP_023624649.1	hypothetical protein	-
EHC70_RS25160	17190..17564	+	375	WP_042761155.1	hypothetical protein	-
EHC70_RS25165	18125..19129	+	1005	WP_158501040.1	Ig-like domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..108287	108287

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-107)

Antitoxin

• Antitoxin PemI-like (1-75)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12159.65 Da
• Isoelectric Point: 5.8124

>T115683 WP_023624650.1 NZ_CP034287:c14275-13934 [Vibrio parahaemolyticus]
MTYAVPEKGDVVVMSFNPQSGHEQAGDSRPGLVISPKEFNQATGFALVCPITNQSKGYPFEVEITGSQKTTGVVLADQVK
SLDWRARRARVVDSVSNETLGTVRDYVTAIANG

Nucleotide

• Length: 342 bp

>T115683 NZ_CP034287:c14275-13934 [Vibrio parahaemolyticus]
ATGACGTATGCAGTACCAGAGAAAGGCGATGTAGTAGTAATGAGTTTTAATCCGCAAAGCGGACACGAACAAGCGGGAGA
CAGTCGGCCTGGGCTGGTCATTTCCCCGAAAGAGTTTAACCAAGCAACTGGCTTTGCTCTGGTATGCCCCATAACCAACC
AGTCAAAAGGGTATCCGTTTGAAGTTGAAATAACCGGAAGCCAAAAAACAACGGGAGTGGTGCTGGCTGACCAAGTGAAG
TCCTTGGATTGGCGCGCGCGTCGCGCTCGTGTGGTAGACTCCGTTTCAAACGAAACACTGGGGACTGTGCGGGATTATGT
CACAGCGATTGCTAATGGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8499.87 Da
• Isoelectric Point: 6.4604

>AT115683 WP_017449238.1 NZ_CP034287:c14502-14272 [Vibrio parahaemolyticus]
MSQTIAKWGHSAAVRIPSKVLKNMDMAVGDKVEVKVFNRQIIIEPAKPTLAQLLSQITDENRHDEVITETMGNELL

Nucleotide

• Length: 231 bp

>AT115683 NZ_CP034287:c14502-14272 [Vibrio parahaemolyticus]
ATGTCACAAACAATAGCTAAGTGGGGCCACAGCGCAGCTGTGCGTATACCTTCTAAGGTATTGAAAAATATGGATATGGC
GGTGGGTGACAAAGTGGAGGTTAAAGTGTTCAATCGACAAATTATCATTGAACCTGCAAAACCAACACTCGCCCAGCTTT
TGAGTCAGATCACTGATGAAAACCGCCATGATGAAGTAATCACCGAAACTATGGGTAATGAATTGTTATGA

Structures

Toxin

• AlphaFold DB: A0A1Y1BA95