Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 75057..75793 Replicon plasmid p72_FIBkpn
Accession NZ_CP034282
Organism Klebsiella pneumoniae strain I72

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag EI552_RS27490 Protein ID WP_003026803.1
Coordinates 75057..75539 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag EI552_RS27495 Protein ID WP_003026799.1
Coordinates 75527..75793 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EI552_RS27470 70607..71569 + 963 WP_004152113.1 M48 family metalloprotease -
EI552_RS27475 71657..72911 + 1255 Protein_77 IS3 family transposase -
EI552_RS27480 73059..73454 - 396 WP_004143398.1 helix-turn-helix domain-containing protein -
EI552_RS27485 73503..74849 - 1347 WP_077253535.1 ISNCY family transposase -
EI552_RS27490 75057..75539 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
EI552_RS27495 75527..75793 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
EI552_RS27500 75969..76223 - 255 WP_004152108.1 hypothetical protein -
EI552_RS27505 76299..76556 - 258 WP_004152107.1 hypothetical protein -
EI552_RS27510 76605..76808 - 204 WP_004152106.1 hemolysin expression modulator Hha -
EI552_RS27515 76842..77210 - 369 WP_004152105.1 hypothetical protein -
EI552_RS27520 77254..77748 - 495 WP_004152104.1 DNA-binding protein -
EI552_RS27525 77779..78354 - 576 WP_004152103.1 hypothetical protein -
EI552_RS27530 78342..78611 - 270 WP_004152102.1 hypothetical protein -
EI552_RS27535 78969..79319 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
EI552_RS27540 79369..79731 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA14 / qnrB1 / tet(A) / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 - 1..193222 193222


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T115679 WP_003026803.1 NZ_CP034282:c75539-75057 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T115679 NZ_CP034282:c75539-75057 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT115679 WP_003026799.1 NZ_CP034282:c75793-75527 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT115679 NZ_CP034282:c75793-75527 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References