Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5352276..5352901 Replicon chromosome
Accession NZ_CP034281
Organism Klebsiella pneumoniae strain I72

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag EI552_RS26665 Protein ID WP_002882817.1
Coordinates 5352276..5352659 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag EI552_RS26670 Protein ID WP_004150355.1
Coordinates 5352659..5352901 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EI552_RS26650 5349642..5350544 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
EI552_RS26655 5350541..5351176 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
EI552_RS26660 5351173..5352102 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
EI552_RS26665 5352276..5352659 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
EI552_RS26670 5352659..5352901 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
EI552_RS26675 5353106..5354023 + 918 WP_009484979.1 alpha/beta hydrolase -
EI552_RS26680 5354037..5354978 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
EI552_RS26685 5355023..5355460 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
EI552_RS26690 5355457..5356317 - 861 WP_004146232.1 virulence factor BrkB family protein -
EI552_RS26695 5356311..5356910 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T115678 WP_002882817.1 NZ_CP034281:c5352659-5352276 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T115678 NZ_CP034281:c5352659-5352276 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT115678 WP_004150355.1 NZ_CP034281:c5352901-5352659 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT115678 NZ_CP034281:c5352901-5352659 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References