Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4080170..4080789 Replicon chromosome
Accession NZ_CP034281
Organism Klebsiella pneumoniae strain I72

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag EI552_RS20450 Protein ID WP_002892050.1
Coordinates 4080571..4080789 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag EI552_RS20445 Protein ID WP_002892066.1
Coordinates 4080170..4080544 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EI552_RS20435 4075322..4076515 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
EI552_RS20440 4076538..4079684 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
EI552_RS20445 4080170..4080544 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
EI552_RS20450 4080571..4080789 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
EI552_RS20455 4080948..4081514 + 567 WP_002892030.1 maltose O-acetyltransferase -
EI552_RS29700 4081486..4081626 - 141 WP_004147370.1 hypothetical protein -
EI552_RS20460 4081647..4082117 + 471 WP_002892026.1 YlaC family protein -
EI552_RS20465 4082092..4083543 - 1452 WP_032435563.1 PLP-dependent aminotransferase family protein -
EI552_RS20470 4083644..4084342 + 699 WP_032435564.1 GNAT family N-acetyltransferase -
EI552_RS20475 4084339..4084479 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
EI552_RS20480 4084479..4084742 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T115673 WP_002892050.1 NZ_CP034281:4080571-4080789 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T115673 NZ_CP034281:4080571-4080789 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT115673 WP_002892066.1 NZ_CP034281:4080170-4080544 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT115673 NZ_CP034281:4080170-4080544 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References