Detailed information of TA system TA115650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3144581..3145215
• Replicon: chromosome
• Accession: NZ_CP034276
• Organism: Pectobacterium versatile strain 14A

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EIP93_RS14240
• Protein ID: WP_103972302.1
• Coordinates: 3144581..3144985 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C6C2Z8
• Locus tag: EIP93_RS14245
• Protein ID: WP_012765080.1
• Coordinates: 3144985..3145215 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EIP93_RS14220	3140498..3141607	-	1110	WP_025919286.1	ISAs1 family transposase	-
EIP93_RS14225	3142523..3142738	+	216	Protein_2766	integrase	-
EIP93_RS14230	3142811..3143919	+	1109	WP_103164902.1	IS3-like element ISEca11 family transposase	-
EIP93_RS14235	3143934..3144263	+	330	Protein_2768	integrase	-
EIP93_RS14240	3144581..3144985	-	405	WP_103972302.1	tRNA(fMet)-specific endonuclease VapC	Toxin
EIP93_RS14245	3144985..3145215	-	231	WP_012765080.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EIP93_RS14250	3145309..3146142	-	834	WP_125232875.1	DUF4942 domain-containing protein	-
EIP93_RS14255	3146261..3146581	-	321	WP_125232876.1	TA system toxin CbtA family protein	-
EIP93_RS14260	3146650..3146985	-	336	WP_125232877.1	type IV toxin-antitoxin system YeeU family antitoxin	-
EIP93_RS14265	3147025..3147498	-	474	WP_102118413.1	DNA repair protein RadC	-
EIP93_RS14270	3147651..3148178	-	528	WP_102118412.1	DUF4234 domain-containing protein	-
EIP93_RS14275	3148236..3148697	-	462	WP_125232878.1	hypothetical protein	-
EIP93_RS22775	3148839..3148997	-	159	WP_161569105.1	hypothetical protein	-
EIP93_RS14285	3149181..3150068	-	888	WP_125232879.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	hcp	3140498..3192851	52353

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15045.26 Da
• Isoelectric Point: 8.0253

>T115650 WP_103972302.1 NZ_CP034276:c3144985-3144581 [Pectobacterium versatile]
MLKYLLDTNICIYTIKNKPQEVRDAFYRHYGQFAISSITLMELIYGAEKSVNPEKNLAVIEGFSARLEVQTYGFDAAVHT
GQIRAELARQGTPIGPYDAMLAGHARSAGLILVTNNVREFERVPGLRVENWVGR

Nucleotide

• Length: 405 bp

>T115650 NZ_CP034276:c3144985-3144581 [Pectobacterium versatile]
ATGCTTAAATATCTGCTCGATACCAATATTTGTATTTACACCATCAAGAACAAACCGCAGGAAGTCAGGGACGCTTTTTA
CCGCCATTACGGGCAGTTTGCTATCAGTTCTATCACGCTGATGGAACTGATCTATGGTGCGGAGAAATCGGTAAATCCAG
AAAAGAATCTTGCCGTGATTGAAGGGTTTTCTGCGCGTCTGGAAGTGCAAACTTATGGCTTTGACGCTGCCGTACATACG
GGGCAAATTCGTGCGGAACTGGCAAGACAAGGAACCCCCATCGGCCCGTATGATGCCATGTTAGCGGGTCATGCGCGGTC
CGCAGGTTTGATTCTGGTAACGAACAATGTGCGTGAGTTTGAGCGTGTGCCAGGATTGCGCGTAGAAAACTGGGTCGGTA
GATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8694.75 Da
• Isoelectric Point: 4.3063

>AT115650 WP_012765080.1 NZ_CP034276:c3145215-3144985 [Pectobacterium versatile]
MEKTTVFKSNRSQAVRLPKAVALPDDVKQVDIVAIGRTRIITPAGESWDSWFEEDGVTPDFMITREQPDDQIREDF

Nucleotide

• Length: 231 bp

>AT115650 NZ_CP034276:c3145215-3144985 [Pectobacterium versatile]
ATGGAAAAAACTACAGTATTTAAAAGCAATCGCAGTCAGGCGGTACGACTCCCTAAAGCCGTTGCACTGCCGGATGATGT
TAAGCAGGTTGATATCGTCGCGATTGGTCGCACACGAATCATTACTCCGGCAGGCGAAAGCTGGGACAGTTGGTTCGAAG
AGGATGGCGTTACGCCTGACTTTATGATCACCAGAGAGCAGCCCGATGATCAGATCAGGGAAGATTTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7V8Q8E4